天麻多糖对天麻钩藤药对效应成分吸收的影响-环球中医药杂志.PDFVIP

摇1452 环球中医药2015年12月第8卷第12期摇 Global Traditional Chinese Medicine,December2015,Vol郾8,No郾12 ·论著 · 天麻多糖对天麻钩藤药对效应成分吸收的 影响 彭尹宣摇 王兴摇 杨娟摇 米佳摇 董雨 揖摘要铱摇 目的摇 研究天麻多糖对天麻钩藤药对效应成分天麻素吸收的影响。 方法摇 采用高效 液相色谱法测定天麻钩藤水煎液组和去多糖天麻钩藤水煎液组中的天麻素在不同时间点大鼠血浆中 的含量;色谱柱:Agilent SB鄄C184.6伊250 mm,5 滋m;流动相:乙腈-0.1%磷酸水溶液(1.6 颐98郾4);检测 波长:220 nm;柱温:30益;流速:1.0 mL/ min;进样量:20 滋L。 结果摇 去多糖组中天麻素的吸收速率 常数(ka)和达峰浓度(C )明显小于天麻钩藤水煎组,而达峰时间(T )比天麻钩藤水煎液组长; max max 天麻钩藤水煎液组中天麻素的半衰期(T )明显比去多糖组长且天麻钩藤水煎组始终维持着较高 1/ 2 的药物浓度,说明在去除天麻多糖后,大鼠对天麻钩藤药对中的效应成分天麻素的吸收效率明显降 低,且天麻钩藤水煎组的曲线下面积(AUC)明显比去多糖组大。 结论摇 天麻多糖对天麻钩藤药对 中的效应成分天麻素的吸收具有促进作用。 揖关键词铱摇 天麻钩藤药对;摇 多糖;摇 天麻素 揖中图分类号铱摇 R285.5摇 揖文献标识码铱摇 A摇 doi:10郾3969/ j郾issn郾1674鄄1749郾2015郾12郾007 Influenceofpolysaccharideoftallgastrodiatuberonabsorptionofeffectivecomponentsinmedicine suits of tall gastrodiatuberand gambirplant nod摇 PENG Yin鄄xuan,WANGXing,YANGJuan,et al. 摇 School of Life Science and Engineering,Southwest Jiaotong University,Chengdu610031,China Corresponding author:WANGXing,E鄄mail:wshing@263.net 揖Abstract铱摇 Objective摇 To study the influence of polysaccharide of tall gastrodia tuber on absorption of gastrodin inmedicinesuitsoftallgastrodiatuberandgambirplantnod. Methods摇 HighPer鄄 formance Liquid Chromatography (HPLC)was used to determine the content of gastrodin in different time points of rat plasma in tall gastrodia tuber and gambir plant nod decoction group (TG group) and tall gastrodia tuber and gambir plant nod without polysaccharide decoction group (TGP group),and with the determination conditions were as following:determination column was Agilent SB鄄C18 4.6伊250 mm,5 滋m;the mobile phasewas consisted with acetonitrile-0.1% phosphoric acid (1.6 颐 98.4);determine wavelength was220 nm;columntemperaturewas30益;fl