多糖高级结构解析方法的研究进展.pdf

PROGRESS IN PHARMACEUTICAL SCIENCES 2015,39 5 :364-369 364 ( ) 多糖高级结构解析方法的研究进展 徐航，朱锐，刘玮，高向东* （中国药科大学生命科学与技术学院，江苏 南京 210009） [ 摘要 ] 研究发现，多糖的生物活性不仅与其一级结构（如分支度、单糖组成及糖苷键连接方式等）有关，而且与其空间结构也密不可分。 因此，对多糖高级结构的研究将有助于阐释其构效关系，为寻找具有生物活性的多糖和开发多糖药物提供理论依据。传统的多糖结构分 析方法具有一定的局限性，已不能满足多糖研究的需求，而现代物理技术的发展则为多糖高级结构解析提供了新的手段。综述近年来多 糖高级结构解析方法的研究进展。 [ 关键词 ] 多糖；高级结构；解析方法 [ 中图分类号 ] Q539；R284.1 [ 文献标志码 ] A [ 文章编号 ] 1001-5094（2015）05-0364-06 Research Progress in Analytical Methods for Advanced Structures of Polysaccharides XU Hang, ZHU Rui, LIU Wei, GAO Xiangdong (School of Life Science and Technology, China Pharmaceutical University, Nanjing 210009, China) [Abstract] It has been found that the bioactivities of polysaccharides are not only dependent on their primary structures, such as branched degrees, monosaccharide compositions and glycosidic linkage modes, but also closely related to their spatial structures. Therefore investigation on advanced structures of polysaccharides could contribute to the elucidation of their structure-activity relationships, and provide theoretical evidence for the screening of bioactive polysaccharides and the development of polysaccharide drugs. Traditional structure analysis methods for polysaccharides can no longer meet the needs of polysaccharides research because of certain limitations, while the development of modern physical techniques has provided new strategies of advanced structure analysis for polysaccharides. The current research progress in analytical methods for advaneed struetures of polysaccharides was reviewed. [Key words] polysaccharide; advanced structure; analytical method 多糖是一类由 10 个以上单糖通过糖苷键而形成的 螺旋结构上，近年来人们发现，硫酸化蛋白聚糖的抗 生物大分子，是构