流式cba常见问题（The common problems of stream CBA）.docVIP

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流式cba常见问题（The common problems of stream CBA）.doc

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流式cba常见问题（The common problems of stream CBA）

流式cba常见问题（The common problems of stream CBA） Common problem： Q: can I use Coulter XL CBA to analyze my data? A: Although CBA kit for BD FACScan? And FACSCalibur? The design of flow cytometry, CBA kit can also use a Coulter XL cell detector features CBA version 1.3 software has the above parameter compatibility, can analyze the experimental data using the instruments of non BD company, please refer to the user guide or software contact technical support to get further information. Q: during the experiment, in addition to the analysis of CBA kit, we also need to prepare for what? A: in addition to included in the kit components, to analyze CBA experiment, also purchased BD trace sample multivariate flow cytometry protein quantitative technical analysis software (cat. No. to 0421CD/ 550065), this software is mainly used to analyze the experimental data of your. Specific software, though the analysis of the experimental data can not use CBA but, due to the acquisition of CBA in the experiment data is more complicated, if not through the analysis of special CBA software is for data analysis of the time will be increased significantly. CBA software is based on Microsoft Excel, users only need to complete the installation of special software MS Excel 98 or Version 5 to install CBA. In addition, users also need a 488 nm laser and 3 fluorescence parameters received by flow cytometry (eg: BD FACScan or BD FACSCalibur cytometer?). Q: the use of CBA in the bead is what to do? What is the average radius of the use of the beads? A: the use of CBA in the bead raw material is polystyrene, about 7.5um. Q: in CBA analysis I should use what type of test tube? A: in addition to FACSArray, we recommend the use of 12x75 mm, 5 ml polystyrene tube. Q: CBA analysis in the preparation of a standard curve, what need to pay special attention to the place? A: standard samples provided by CBA kit in lyophilized protein is sensitive, because most of the protein standard sample belongs to the clas