薄层色谱扫描法测定海康胶囊中海参多糖含量（Determination of polysaccharide content in Kang Capsule by TLC scanning）.docVIP

薄层色谱扫描法测定海康胶囊中海参多糖含量（Determination of polysaccharide content in Kang Capsule by TLC scanning） Determination of polysaccharide content in Kang Capsule by TLC scanning Update: 2011-03-06 Li Haitang Zhou Qingkai Cai Yunjian Zhang Suifang The determination of polysaccharide content in Kang capsule was 51.4% by agar electrophoresis scanning method. Keywords sea cucumber polysaccharide; agar electrophoresis; chromatography; Determination of content Sea cucumber in our country folk had been used to treat tuberculosis, impotence, aplastic anemia, diabetes, hepatitis and other diseases. We have reported using sea cucumber polysaccharide as the main raw material of Kang capsule and Mieaoling were used in the treatment of chronic hepatitis B, to obtain a certain effect (Zhou Qingkai, Cai Yun, Zhang Lingchu, et al. Isolation and treatment of hepatitis B polysaccharide from sea cucumber preliminary clinical observation of.1994 in Lanzhou national polysaccharides and lipids biochemical medicine academic conference proceedings, page 92). In order to control the quality of clinical medicine, conducted a preliminary study on the determination of contents of sea cucumber polysaccharide. The determination method of the report is as follows. 1 instruments and materials SHIMADZU CS-930 dual wavelength TLC scanner; agarose electrophoresis with biochemical reagent; chemical reagents were of analytical grade; sample test solution (Kang capsule), sea cucumber polysaccharide liquid control (homemade). Methods and results 2 2.1 sample sample preparation: Kang capsule 5, pour out the contents of blending, precision weigh 0.1 g content, 20 ml 30 min hot water, filtering the insoluble, repeated with 20 ml water 30 min hot, filtering, merging filtrate volume is determined as 50 ml (2 mg/ml) sample test solution. 2.2 sea cucumber polysaccharide control liquid preparation: weigh accurately sea cucumber polysaccharide control (HA-P) (homemade, see below), heated and dissolved, water filtration, prep