紫外光耐受性细菌筛选与鉴定 - 建国科技大学.doc

紫外線輻射耐受型土壤細菌之篩選與鑑定 Isolation and Identification of an Ultraviolet-Radiation-Tolerance Bacterium from Soil 蕭蓉禎 Rong-Jen Shiau 建國科技大學美容系 Department of Beauty Science, Chienkuo Technology University 蕭蓉禎 博士 建國科技大學美容系 彰化市介壽北路號 Tel: 04-7111111 ext Fax: 04-7111118 E-mail: rjshiau@.tw 摘要 紫外線輻射照射皮膚後，會造成皮膚老化、產生皺紋、以及黑色素沉澱等變化。長時間暴露於紫外線輻射下，更會增加罹患皮膚癌的風險。因此，防曬保養品成了保護皮膚對抗紫外線輻射的一種重要工具。本研究的目是篩選出對紫外線輻射具有耐受性的土壤菌株，用以研發新的抗紫外線輻射防曬商品。在55個受測的土壤樣品中，有一個樣品含有紫外線輻射耐受性的菌群接著，在菌群中隨機挑選3個單一的菌落，培養後進行菌種鑑定。經由比對其16S rRNA基因序列，發現這3個菌株均與假單胞桿菌屬的細菌非常相似（99 % 相似度）。親源關分析顯示此3個菌株與假單胞桿菌屬的螢光假單胞桿菌Pseudomonas fluorescens）分在同一群。因而，將此具有紫外線輻射耐受性的菌株命名為Pseudomonas sp. UVT8。未來，可進一步探討Pseudomonas sp. UVT8對抗紫外線輻射傷害的機制，並評估其作為新的防曬商品的潛力。 關鍵詞：紫外線輻射耐受型細菌、親源關、土壤、篩選 Abstract Ultraviolet radiation can cause skin aging, wrinkling, and pigmentation. Prolonged exposure to UV radiation increases the risk of skin cancer. Sunscreen products, therefore, have become an important tool for protection skin damage resulting from exposure to UV light. The aim of this study was to discover soil bacteria which are resistant to UV-dependent damages and can be used for developing new sunscreen products against UV radiation. One out of 55 soil samples contained a group of bacteria which have tolerance to UV radiation. Three randomly selected isolates from this group of bacteria were subjected to species identification. The results based on 16S rRNA gene sequences showed that all three isolates are similar to Pseudomonas (99% similarity). Phylogenetic analysis demonstrated that these three isolates were clustered with Pseudomonas fluorescens. Therefore, this UV-radiation- tolerance bacterium was named as Pseudomonas sp. UVT8. It is worthy to further investigate the mechanism of UV- radiation-tolerance of Pseudomonas sp. UVT8, and to evaluate Pseudomonas sp. UVT8 as a new potential sunscreen product. Key words: ultraviolet-radiation-tolerance bacterium, phylogeny, soil, screening 壹、前言 輻射泛指具有能量的電磁波或高速粒子。自然界的輻射來源有礦物、宇宙射線及太陽等。在這些輻射來源中，太陽輻射對於維繫地球生態系統平