亚抑菌浓度头孢他啶对临床尿管分离大肠埃希菌 - 第三军医大学学报.docVIP

亚抑菌浓度头孢他啶对临床尿管分离大肠埃希菌 生物膜的作用 欧倩怡1，何召云2，夏培元1，高宇3，孙凤军1 （400038 重庆，第三军医大学西南医院：药剂科1，泌尿外科3；100039 北京，解放军302医院肝硬化诊疗中心2） [摘要] 目的 研究亚抑菌浓度头孢他啶对临床尿管分离大肠埃希菌生物膜形成的作用，为临床抗菌药物的合理使用提供理论依据。方法 采用琼脂平板倍比稀释法检测大肠埃希菌的最低抑菌浓度，双纸片协同法检测大肠埃希菌产超广谱β-内酰胺酶的能力，菌落计数法分析细菌的粘附能力，96孔板结晶紫染色法检测细菌生物膜的形成。结果 临床分离大肠埃希菌对氨苄西林、头孢哌酮、头孢噻肟和哌拉西林具有较高的耐药性，对阿米卡星、亚胺培南和美洛培南全部敏感。大肠埃希菌ESBLs阳性率为73.3%。亚抑菌浓度头孢他啶可以抑制临床尿管分离大肠埃希菌的粘附和生物膜形成。结论 头孢他啶可抑制大肠埃希菌生物膜形成，但其抑制程度具有菌株差异性。 [关键词] 大肠埃希菌；亚抑菌浓度；头孢他啶；生物膜；尿管( Effect of sub-minimal inhibitory concentration ceftazidime on biofilm formation of Escherichia coli isolated from urethral catheters Ou Qianyi1, He Zhaoyun2, Xia Peiyuan1, Gao Yun3, Sun Fengjun1 (1Department of Pharmacy, 3Department of Urology, Southwest Hospital, Third Military Medical University, Chongqing, 400038; 2. Liver Cirrhosis Diagnosis and therapy Center, 302 Hospital of PLA, Beijing, 100039, China) [Abstract] Objective: To study the effect of sub-minimal inhibitory concentration (sub-MIC) ceftazidime on biofilm formation of Escherichia coli isolated from urethral catheters and provide the rational information to use antiobiotics reasonably. Methods: The minimal inhibitory concentration was determined by the agar double dilution method. Extended-spectrum β-lactamases (ESBLs) were detected by double-disk synergy method. The adhesion of E. coli was analyzed using flat colony counting method, and the biofilm formation was assayed using the 96-well crystal violet staining method. Results: E. coli strains isolated from urethral catheters had high resistance to ampicillin, cefoperazone, cefotaxime and piperacillin and were fully sensitive to amikacin, imipenem and meropenem. The ESBL-producing E. coli strains accounted for 73.3%. The sub-MIC ceftazidime could inhibit the adhesion and biofilm formation of E. coli isolates. Conclusion: Ceftazidime could inhibit the biofilm formation of E. coli, but its inhibition had differences between the strains. [Key words] Escherichia coli; Sub-minimal inhibitory concentration; Ceftazi