松油烯-醇对粘虫几种代谢酶及酚氧化酶的影响.docVIP

松油烯-4-醇对粘虫几种代谢酶及酚氧化酶的影响 马志卿，韩秀玲，冯俊涛，李广泽，张 兴 （陕西省生物农药工程技术研究中心/西北农林科技大学无公害农药研究服务中心，陕西杨凌 712100） 摘要：【目的】探讨杀虫植物砂地柏Sabina vulgaris精油中主要杀虫成分松油烯-4-醇的作用机理。【方法】以点滴法处理粘虫5龄幼虫后，测定兴奋期、痉挛期、麻痹期及复苏期等4个中毒阶段试虫体内4种代谢酶及酚氧化酶（PPO）的活性。【结果】松油烯-4-醇仅在麻痹期对酸性磷酸酯酶（ACP）具有一定的抑制作用，而在其它时期均可明显激活ACP及碱性磷酸酯酶（AKP）；对谷胱甘肽S转移酶（GSTs）则表现出先抑制后恢复的趋势；可显著抑制细胞色素P450 O-脱甲基活性，各中毒阶段酶抑制率分别为26.27%、46.03%、80.24%和90.22%；离体和活体条件下，均可显著抑制PPO活性。【结论】松油烯-4-醇对粘虫代谢酶系细胞色素P450、GSTs及PPO的抑制与其毒杀活性有关，而中毒试虫的复苏可能与GSTs活性的恢复有关。该化合物可开发为新型农药或增效剂，在害虫抗性治理中具有潜在的应用价值。 关键词：松油烯-4-醇；粘虫；酚氧化酶；代谢酶；作用机理 Effects of Terpinen-4-ol on 4 kinds of Metabolizing Enzymes and PPO in Mythimna separta Walker MA Zhi-qing, HAN Xiu-ling, FENG Jun-tao, LI Guang-ze, ZHANG Xing (Shaanxi Research Center of Biopesticide Technology and Engineering/Biorational Pesticide Research and Development Center of Northwest Agriculture and Forestry University, Yangling 712100, Shaanxi) Abstract: 【Objective】To study insecticidal mechanism of terpinen-4-ol, the main insecticidal compo in essential oil of Sabina vulgaris. 【Method】The 5th instar larvae of the Mythimna separta were treated by terpinen-4-ol with topical application. The activities of phosphatase, glutathione S-transferase GSTs), cytochrome P450 (P450) and polyphenol oxidase (PPO) of insects were determined at all poisoning stages. 【Result】The activities of both acid phosphatase (ACP) and alkaline phosphatase (AKP) in insects were induced by terpinen-4-ol, but ACP was inhibited paralysis stage. The activities of GSTs were inhibited at exciting stage, convulsing stage and paralysis stage, but gradually recovered at recovering stage. O-demethylase activity of cytochrome P450 was inhibited by terpinen-4-ol, the inhibitory rate at all poisoning stages was 26.27%, 46.03%, 80.24% and 90.22%, respectively. PPO activities were strongly inhibited by terpinen-4-ol, both in vitro and in vivo.【Conclusion】 The activities of P450, GSTs and PPO probably relations with toxicity of terpinen-4-ol against larvae of the Mythimna separta,