同源导入近交系绿色荧光裸小鼠-中国试验动物学报.docVIP

同源导入近交系绿色荧光裸小鼠 Foxn1nu.B6-CAG-EGFP/SU的建立 沈艳华1，王麒龙2，代兴亮2，陈金2，董军2，兰青2，黄强2 （1. 苏州大学医学部实验动物中心,苏州，215123；2. 苏州大学附属第二医院神经外科，苏州，215123） 【摘要】 目的 ? 建立一个能稳定表达增强型绿色荧光蛋白(Enhanced Green Fluorecence Protein，GFP)的裸小鼠近交新品系，供包括人脑胶质瘤在内的所有人类肿瘤移植实验用。方法 将C57BL/6-Tg(CAG-EGFP)转基因小鼠和Foxn1nu裸小鼠，按同源导入方法进行杂交和回交，用荧光手电筒和匹配的眼镜、多功能活体成像仪、荧光显微镜对是否表达EGFP进行鉴别，传至F10后进行遗传学检测。结果 所建品系命名为 Foxn1nu.B6-CAG-EGFPSU (Soochow University)，14个遗传生化位点中13个表型与BALB/c（Foxn1nu）吻合，唯Pep3（肽酶-3）的电泳为b型,而标准的BALB/c近交系为a型;外周血淋巴细胞占全部有核细胞15%，T淋巴细胞仅占0.3%，与亲本的Foxn1nu一致。结论 成功地建立了一个既与亲本C57BL/6-TgN(CAG-EGFP)－样稳定表达EGFP，又与Foxn1nu裸小鼠（BALB/c背景）一样具有T细胞缺乏的荧光裸小鼠新品系- Foxn1nu.B6-CAG-EGFP｜SU ,Pep3为b型是有别于Foxn1nu裸小鼠的生化遗传标记位点。 绿色荧光蛋白；转基因小鼠；裸小鼠；近交系 【中图分类号】R33【文献标识码】A 【文章编号】 Establishment of a new congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU SHEN Yan-hua, WANG Qi-long, DAI Xing-liang, CHEN Jin-sheng, DONG Jun, LAN Qing, HUANG Qiang ( 1. Laboratory animal center of Soochow University, Suzhou, 215123, China；2. The Second Affiliated Hospital of Soochow University, Suzhou,215123,China) 【Abstract】Objective To establish a new congenic inbred mouse strain carrying and expressing EGFP and Foxn1nu gene for cancer research including human glioma as well. Methods According to criterion of GB14923-2010, the male Foxn1nu nude mice backcross the female C57BL/6-Tg (CAG-EGFP) transgenic mice for 10 times, Then identify the phenotype using the methods and equipment as below: fluorescent flashlight and matching glasses; multifunction vivo imager; fluorescence microscopy. Results The congenic inbred mouse strain named Foxn1nu.B6-CAG-EGFP/SU (Soochow University). All the 14 biochemical loci are homozygous and same with Balb/c mouse in addition to the Pep3 loci (“b” type instead of “a” type). Peripheral blood lymphocyte count shows the lymphocytes occupy 15% of nucleated cells; T lymphocytes occupy 0.3%, meet the requirement of inbred strain of EGFP nude mice. Conclusions Established a new congenic inbred strain -Foxn1nu.B6-CAG-EGFP/SU which both expr