DPA对细胞壁缺陷枯草芽胞杆菌耐热性影响的研究.pdfVIP

· 8 · Guizhou Medical Journal，2008，Vo1．32，No．1 DPA对细胞壁缺陷枯草芽胞杆菌耐热性 影响的研究 贵阳医学院微生物学教研窒(550004) 王 涛 王 和▲ 捅 要 目的 探讨吡啶二羧酸(DPA)对细胞壁缺陷枯草芽胞杆菌耐热性的影响。方法 用 内酰胺抗生素诱导枯草芽胞杆菌(Bacillus subtilis)成为L型并传代培养获得稳定L型纯培养物， 采用形态学方法、紫外分光光度法及热稳定性试验检测稳定L型的芽胞、DPA及耐热性。结果在稳 定L型不能发现典型形态的芽胞，但可检测到DPA合成代谢活性以致DPA在茵细胞内大量堆积。 L型细胞内虽然存在有大量的DPA，但却不能增强其对高温杀菌的抵抗力。结论枯草芽胞杆菌稳定 L型丧失形成芽胞的能力，但能合成DPA。然而稳定L型繁殖体细胞内的DPA并不能增强茵细胞 对热的耐受能力，DPA增强蛋白质热稳定性的反应需要在芽胞内进行和完成。 关键词 吡啶二羧酸 耐热性 枯草芽胞杆菌 细菌L型 studv of the Effect on the DPA to Heat Stability of Cdl W l ficiency Bacillus subtilis％ ng Tao，Wang He．DepartmentofMicrobiology，GuiyangMedical College，550004 Abstract Objective To probe the effect on the DPA tO heat stability of eell wall deficiency Bacil— lUS subtilis．Methods The L-forms of Bacillus subtilis was induced by lactam antibiotic and then the pure cultures of stable L-form were isolated and obtained by SUbculturing on the LEM The sporula— tion，biosynthesis of DPA and heat stability of the stable L-form cells were determined by the methods of morphology，photochemistry and heat stability tesL Results The spore could not be found in the sta— ble L-forrn cells，but synthesis of the DPA could be determined in L-form cells SO that the DPA were synthesized and accumulated in the stable L-form cells．The DPA could not inerease the heat stability of stable L-foFITI cells Condusion The mechanism of the DPA synthesis would be promoted following the eell wall deficiency in stable L-form cells．But the DPA did not increase stability of the L-forlTl veg— etative cells tO heating beside in the spore． Key words DPA Heat stability Bacillus subtilis L-form of bacteria 中图分类号：R37 文献标识码：A 文章编号：1000—744X(2O08)O卜0008-03 细菌的芽胞(spore or endospore)对热、辐射、 导成为L型，并采用形态学、化学分析、生理学的方 干燥、酸、碱、有机溶剂及抗菌药物等理化因素具有 法，研究DPA对不产生芽胞的稳定L型繁殖体细 比繁殖体显著强的抵抗力，在自然环境条件下能保 胞耐热性的影响，现将结果报告如下。 持生命数百年甚至