DNA修复基因XRCC1单核苷酸多态性与铂类药物化疗对外周血白细胞的影响相关性分析.pdfVIP

实用癌症杂志2008年3月第23卷第2期 The Practical Journal of Cancer，March 2008．Vol 23．No．2 DNA修复基因XRCC1单核苷酸多态性与铂类药物化疗对 外周血白细胞的影响相关性分析 王宜宗 张军宁 王建平 陈 彬 【摘要】 目的 探讨x射线损伤修复交叉互补基因1单核苷酸多态性与应用铂类药物化疗前后外周血白细胞变 化的关系。方法 化疗前采集外周静脉血，用多聚酶链反应一高温连接酶反应(PCR—LDR)进行XRCC1 Arg399Gln基因 多态性的分型；观察化疗前后白细胞计数改变的情况。结果 XRCC1 Arg399Gln各基因型与化疗后白细胞总数、中性粒 细胞计数下降无相关性(P0．05)；携带XRCC1 399Arg／Gln及Gln／Gln基因型的患者化疗后淋巴细胞计数低于Arg／Arg 者，而淋巴细胞下降的相对数要高于Arg／Arg者，化疗淋巴细胞下降绝对值无显著性差异。结论 XRCC1 399Arg／c1n基 因型与化疗后淋巴细胞下降相关，在一定程度上可以预测化疗后淋巴细胞减少的程度。 【关键词】 x射线损伤修复交叉互补基因1；基因多态性；化学治疗 中图分类号：R329．2 文献标识码：A 文章编号：1001—5930(2008)02一o119—03 The Relationship between Polymorphisms of DNA X-ray Repair Cross Complementary Protein l and the Changes of White Blood Cell after Platin-Based Chemotherapy WANG Yi—zong，ZHANG Jun—ning，WANG Jian-ping，et a1． Department of Radiotherapy in the First Hospital Affiliated tO Suzhou University，Suzhou，215006 【Abstract】 Objective To investigate the relationship between genetic polymorphisms of DNA X—ray repair cross comple— mentary protein 1(XRCC1)and the changes of white blood cell(WBC)after platin—based chemotherapy．Methods Peripheral venous blood was used to determine the polymorphisms of XRCC1 codon Arg399Gln by polymerase chain reaction and ligase de— tection reaction f PCR—LDR 1．WBC coUnts were performed before and after chemotherapy，Results No significant difference of the WBC and neutrophil counts after chemotherapy was discovered between the XRCC1 codon Arg399Gln genotypes．The lympho— cyte counts in the cases with 399Arg／Gln or Gln／G1n was significantly lower than that with Arg／Arg after chemotherapy．The rel— ative number of lymphocyte decrease in the eases with 399Arg／Gln or Gln／Gln was significantly more than that with Arg／Arg，but there was no significant difference in the absolute number of decrease of lymphocyte am