Morphological and molecular characterization of Alternaria isolates on fruits of Pyrus bretschneideri Rehd. ＂Ya Li＂.pdfVIP

Mycosystema 菌 物 掌 报 1 5 Janua~2008。27(1)：105—11 7 jwxt@im．ac cn ISSN1 672—6472 CN1 1—51 80O ~2008 Institute of Microbiology，CAS，all rights resewed MorphoIogiCaI and molecular characterization of A，ternaria isolates on fru its of S bretschneideri Rehd． “Ya Li” SUN Xia 1,2 ZHANG Tian—Yu Department ofPlant Pathology,Shandong Agricultural University,Tai’an 271018,China 2Department oflnformation Engineering,Laiwu Vocational Collegefor Technology,Laiwu 271100,China Abstract：A total of 1 23 Alternaria isolates were obtained from roting fruits of Pyrus bretschneideri‘Ya Li’’ collected in Hebei Province，China．The isolates，according to morphological characteristics of conidia and sporulation paaerns，were segregated into four groups：A．alternata group，A．tenuissima group，A．yaliinficiens group and Alternaria sp．group．Molecular characteristics of part of the isolates were determined using random amplified polymorphism DNA(RAPD)analysis．Based on cluster analysis of RAPD data，large-and small—spored Alternaria spp．were evidently distinguished，and the small-spored species Can form five clusters that fundamentally paralleled the morphological groupings． Key words：Alternaria yaliinficiens，fruit rot，RAPD，taxonomy l l ’I’RoDUC’I’lo Alternaria spp．are one kind of the most coin[non fungal pathogens of pear causing defoliation， fruit rot and fruit drop ofthe host plants．Nagano(1920)validly described a fungus causing ring—spot disease ofpear in Japanese and named it Alternaria gaisen Nagano．Later,Tanaka(1933)reported his results of the study on black spot disease of Japanese pear“(Pirus serotina Rehd．)”，emphasizing physiological and cultural characters of the causal fungus，and described A．kikuchiana Tanaka． Simmons studied earnestly the two nam es and pointed out that Tanaka’S description，in English and Corresponding author．E-mail：tyzlmng1937@yahoo．corn．cn Received：16-03-2007，accepted：26