脉冲Nd：YAG激光照射剂量对口腔溃疡组织中NO及NOS含量的影响.DOCVIP

脉冲Nd：YAG1，　刘鲁川1﹡， 刘娜1， 贾文东2 （1第三军医大学大坪医院野战外科研究所口腔科2，重庆4000042；2兰州军区司令部门诊部口腔科，兰州730000） 【摘　要】　目的：探讨脉冲Nd:YAG激光不同照射剂量对大鼠试验性口腔粘膜溃疡组织中一氧化氮（NO）及一氧化氮合酶（NOS）含量的影响。方法：健康Sprague-Dawley大鼠56只，随机分为7组，1组为正常对照组，6组建立口腔粘膜溃疡动物模型。按照分组给予不同剂量激光照射，末次治疗24 h后观察溃疡愈合时间。采用分光光度仪测定大鼠口腔粘膜组织中NO、NOS的含量。结果：试验剂量范围内，1.5 w 60 mJ、1.5ｗ 80 mJ激光组较溃疡组NO、NOS活性显著降低（P 0.05））on the changes of Nitric Oxid and Nitric Oxide Synthase content in oral ulcer tissue Yang Su-ping1，Liu Lu-chuang1﹡，Liu Na1，Jia Wen-dong2 (1Department of stomatology ，Institue of field surgery ，Daping Hospital，the Third Military Medical University，Chongqing 400042，China ; 2Department of stomatology ，Out-patient department, headquarters, a military area of lanzhou,lanzhou 730000) [Abstract] Objective: To observe the effect of different energy levels of pulsed Nd:YAG laser irradiation on the changes of NO and NOS levels in ulcer tissue of oral ulcer model.Methods:56 Sprague-Dawley rates were randomly assigned to 7group,1group randomly assigned as the control Group,the rest 6 group establish the animal model of oral ulcer. Different energy levels of pulsed Nd:YAG laser irradiation on each group, ulcer hesaing times were detected 24 hours after the last treatment.The changes of NO and NOS levels in the oral cavity mucosa tissue which were assayed by spectrophotomentry. Results: Level of NO and NOS activity in 1.5w 60mJ、80mJ groups was evident to lower than the ulcer group (p 0.05 ) ; but he changes of NO and NOS activity was no significant difference ( P 0. 05) between the1.5w 60mJ、80mJ groups and the healthy group. decreased the NO activity and NOS content it is a effective iatreusis to oral cavity mucosa ulcer. [ Key words] pulsed Nd:YAG laser irradiation oral ulcer NO NOS 口腔粘膜病病种多，许多疾病无特异的治疗方法，治疗效果欠佳[1]。由于口腔环境特点，全身用药，费用大且毒副作用多，局部用药不方便且很难保证药物的浓度。90年代初，国外研制成功口腔科专用的 Nd：YAG激光机，其光纤直径0．2～0．4mm，纤细、柔韧、容易达到口腔内任何部位[]，操作简便，调节灵活，可根据不同的临床需要进行能量设置，对许多口腔粘膜病均有缓解疼痛、促进愈合的作用。NO作为一种细胞信使分子和细胞毒性因子，上皮细胞通过合成NO可以限制微生物及其毒性产物侵入上皮及上皮下结缔组织，从而增