三拗汤主要功效成分多靶点网络构建-天津中草药杂志社.PDFVIP

·3370 · 中草药 Chinese Traditional and Herbal Drugs 第46 卷 第22 期 2015 年11 月 三拗汤主要功效成分多靶点网络构建 刘 洪 1, 2 ，范欣生 1* 1. 南京中医药大学基础医学院，江苏 南京 210023 2. 南京中医药大学 图书馆，江苏 南京 210023 摘 要：目的 采用网络药理学方法对三拗汤治疗哮喘的功效成分及作用机制进行分析，探讨多成分-多靶点-多通路的网络 关系。方法 检索近20 年文献中治疗哮喘疗效确切的方剂，分析具有代表性配伍关系组方的功效成分，依据PharmMapper 数据库构建多成分-蛋白网络，获取的靶点信息利用博奥数据库MAS3.0 系统得到相应通路，通过Cytoscape 软件建立成分- 靶点-通路网络模型。结果 文献检索发现三拗汤组成麻黄、杏仁、甘草为现代哮喘方剂中主要的配伍。通过成分-通路-靶点 网络分析表明，三拗汤 11 个主要功效成分作用于76 个靶点，涉及免疫- 内分泌-信号传导-细胞过程中如T 细胞受体、PPAR 、 ErbB 等46 条通路。结论 三拗汤组成药物的功效成分既有共同的作用靶点及通路，又各有侧重，为明确药物之间的配伍关 系和探讨哮喘方剂的作用机制提供了参考。 关键词：三拗汤；功效成分；哮喘；靶点；网络药理学 中图分类号：R285.5 文献标志码：A 文章编号：0253 - 2670(2015)22 - 3370 - 07 DOI: 10.7501/j .issn.0253-2670.2015.22.014 Multi-targets network construction of main effective compounds of San-ao Decoction LIU Hong1, 2, FAN Xin-sheng1 1. Basic Medical College of Nanjing University of Chinese Medicine, Nanjing 210023, China 2. Library of Nanjing University of Chinese Medicine, Nanjing, 210023, China Abstract: Objective To explore the effective components and mechanism of San-ao Decoction for treating asthma by network pharmacology study, and to investigate the correlations among multi-compounds, multi-targets and multi-pathways. Methods Effective formulaes for asthma in which effect index had been tested were searched in the past 20 years ’ literature. Major active compounds from the formulae consisted of Chinese medicines with representative compatibility relation were analyzed. The components-proteins networks based on PharmMappear database were built. The targets information access was used to construct and visualize components-targets-pathways network model using Boao pathway database and Cytoscape software. Results and Conclusion The results showed that Ephedra sinica , Armeniacae Amarum Semen, and Glycyrrhiza uralensis which consisted San-ao Decoction were the major compatibility in formulaes f