不同生长物质复配处理对烤烟种子萌发的影响.PDF

20 Chinese Tobacco Science 2014-04 ，35 （2 ） 1,2 2 1,2 3 3 1,2* 1. 266101 2. 266101 3. 266101 MS GA3IBA 6-BA 4 K326NC89 2 1/20 MS+100 mg/L GA +2 mg/L IBA+0.2 mg/L 6-BA 1/20 MS+100 mg/L GA +2 mg/L IBA+2 mg/L 6-BA 3 3 WRKY2 S572.041 1007-5119201402-0020-06 DOI 10.13496/j.issn.1007-5119.2014.02.004 Effects of Several Growth Substance Combination Treatment on Priming of Flue-cured Tobacco Seeds 1,2 2 1,2 3 3 1,2* QU Xu , DING Anming , LIU Shaoyun , LV Guoxin , WANG Yongli , LI Yijun (1. Qingdao Tobacco Seed Co., Ltd., Qingdao 266101, China; 2. Tobacco Research Institute of Chinese Academy of Agricultural Sciences, Qingdao 266101, China; 3. Tobacco Company of Qingdao, Qingdao 266101, China) Abstracts: To improve the quality of primed tobacco seeds, especially the germination ability of old seeds, studies were conducted on the seeds of K326 and NC89 of different years. The seeds were primed by different concentration of MS medium, GA3, IBA and 6-BA, and then the germination rate, physiological and biochemical indices and the expression of germination related gene were detected. The results showed that after priming treatment by proper concentration of each factor, the germination rate of flue-cured tobacco seeds were promoted, and the combination of different growth regulators (1/20 MS+100 mg/L GA3+2 mg/L IBA+0.2 mg/L 6-BA and 1/20 MS+100 mg/L GA3+2 mg/L IBA+2 mg/L 6-BA) had the best effects, After treatment of which the seeds geminated rapidly and each physiological and biochemical index was b