蓬累悬钩子组织培养及试管苗繁殖探究.docVIP

蓬累悬钩子组织培养及试管苗繁殖探究摘 要：为保护野生资源和实现人工栽培，以蓬累悬钩子的冬眠芽为材料，采用组织培养的方法，进行了冬眠芽伸长生长、分化，伸长生长芽和不定芽生根，试管苗的移栽和定植的研究。结果表明，MS+IAA 0.1 mg·L-1+GA3 0.5 mg·L-1+6-BA0.5 mg·L-1是冬眠芽伸长生长培养的理想培养基；MS+NAA 0.1 mg·L-1+ZT1.2 mg·L-1是伸长生长芽的茎段分化不定芽培养的理想培养基；1/2MS+NAA0.1 mg·L-1+IAA0.2～0.4 mg·L-1是伸长生长芽和不定芽生根培养的理想培养基；试管苗移栽成活率为91.6%，定植成活率为98.2%。定植的试管苗保持了蓬累悬钩子的生物学性状，翌年开花结果。建立起蓬累悬钩子试管苗的繁殖技术。 关键词：蓬累悬钩子；组织培养；试管苗 中图分类号： S663.2 文献标识码：A DOI编码：10.3969/j.issn.1006－6500.2012.04.003 Tissue Culture and Propagation of Tube Seedlings of Rubus crataegifolius YANG Liu，LI Zhi-dong，ZHANG Chen，YANG Wen-xin，JIANG Chang-yang (College of Life Science, Liaoning Normal University, Dalian, Liaoning 116081, China) Ａｂｓｔｒａｃｔ: The aim was to preserve the wild resources and meet the needs of false planting, with the method of tissue culture, dormant buds of Rubus crataegifolius were used as material to do the research on elongation and differentiation of dormant buds, rooting of elongation growth and adventitious buds, transplantation and stable planting of tube seedlings. The results showed that MS + IAA 0.1 mg·L-1 + GA3 0.5 mg·L-1 + 6-BA0.5 mg·L-1 was the ideal medium for elongation of dormant buds;MS + NAA 0.1 mg·L-1+ZT1.2 mg·L-1 was the best medium for adventitious buds differentiation of elongation growth buds’ tender stems; 1/2MS + NAA0.1 mg·L-1 + IAA0.2 ～0.4 mg·L-1 was suitable for rooting of elongation growth and adventitious buds; the transplanting survival rate of tube seedlings was 91.6% and stable planting survival rate was 98.2%.The tube seedlings transplanted maintained all biological characteristics of Rubus crataegifolius and beared following year. The propagation technology of tube seedlings of Rubus crataegifolius was established. Key words: Rubus crataegifolius; tissue culture; tube seedlings 蓬累悬钩子（Rubus crataegifolius）又称牛迭肚、山楂叶悬钩子、托盘、婆婆头等，属于蔷薇科、悬钩子属落叶灌木，生长于山坡灌丛或林边，在我国的东北、河北、内蒙古和山东等地有分布[1]，在辽宁的沈阳、大连、鞍山和丹东等地也有分布[2]。蓬累悬钩子的茎皮可造纸，也可做纤维，其果实既可鲜食，又可以制成果酱和果酒。鲜果中含丰富的SOD（超氧化物歧化酶）等成分，具有醒酒、止咳、祛痰、解毒、抗癌、抗衰老等功效，能治痛风