化学染毒及高温环境对孕鼠子代海马神经元形态及神经行为的影响.docVIP

化学染毒及高温环境对孕鼠子代海马神经元形态及神经行为的影响 张 红，赵聪敏，张雨平，王文益（第三军医大学新桥医院儿科，重庆 400042） 摘 要：目的 探讨化学毒物及高温环境对孕鼠子代海马神经元的形态和神经行为的影响。方法 将孕鼠随机分为对照组、染毒组、染毒联合高温组高温组1－10）染毒组和联合组海马CA1区变性坏死的神经元数量明显多于对照组和单纯高温组（P0.01）。出生一月后染毒组海马CA1神经元的数量明显少于对照组和单纯高温组（P0.0）染毒组、联合组高温组早期反射出现的时间均明显长于对照组染毒联合高温并不加重脑组织海马神经元的损害，高温对动物的早期反射却有明显的延迟作用。Effects of chemical compounds exposure and hyperthemial environment to pregnant rats on hippocampus neuronal morphology and neurobehavioral development of offsprings ZHANG Hong, ZHAO Chong-min, ZHANG Yu-ping, WANG Wen-yi (Department of Pediatrics, Xinqiao Hospital, Third Military Medical University, Chongqing 400042, China) Abstract：Objective To observe the effects of chemical compounds exposure and hyperthemal environment to pregnant rats on the hippocampus neuronal morphology and neurobehavioral development of their offsprings. Methods Pregnant rats were divided into 4 groups randomly: the control group; chemical compounds exposure group, chemical compounds combined hyperthemial exposure group and hyperthermal group. The control group was living in normal condition, the chemical compounds exposure group in a cabin with benzene (20.26±0.80) mg?m-3, toluene (39.66±4.23) mg?m-3, dimethylbenzene (42.40±2.85) mg?m-3, and formaldehyde (23.13±1.30) mg?m-3, the hyperthermal group was kept in an atmosphere of 38.5℃, and the combined group was exposed to the chemical compounds and high temperature at the same time. All animals were treated respectively 2 h per day for 10 d since their pregnancy. Their offspring were lived in normal condition. The development of brain and changes of hippocampus neuronal morphology were observed just or 1 month after born. The primary reflex activity of new-born rats was also examined. Results There was no abnormality in the brain of new born rats from 4 groups. In the chemical compounds exposure and the combined exposure groups, the number of degeneration and necrosis neurons in CA1 region of hippocampus were significantly more than those in control and