rhvdm–afastanduser-friendlyrpackageto-videolectures.pptVIP

Dynamic modelling of microarray data. Martino Barenco Institute of Child Health / UCL Goal: predict targets of a known transcription factor in a complex response using dynamic models and time course microarray data. HVDM: Hidden Variable Dynamic Modelling Gene expression model The p53 network Experimental setup Human T cells (MOLT4/p53 wild-type) submitted to 5Gy irradiation. mRNA harvested 2,4,6,8,10,12 hours after irradiation, and just before (0 hrs time point). Affymetrix microarrays (HG-U133) were then run. Experiment was run in triplicates. Results of training step: activity profile of