35低β-葡萄糖苷酶纤维素酶制备纤维低聚糖-南京林业大学毕业设计.doc

南京林业大学 本科毕业设计（论文） 题 目：低聚木糖生产废渣制备纤维低聚糖的研究 学 院： 化学工程学院 专 业： 生物工程 学 号： 070204221 学生姓名： 吴 飚 指导教师： 勇 强 职 称： 教 授 二O一O年 月 日  摘要研究温度、pH、时间对低聚木糖生产废渣吸附纤维素酶各组分的影响优化的吸附条件是温度10℃、pH7.0、30min，内切葡聚糖酶吸附率87.67%，β-葡萄糖苷酶吸附率39.74% ，内切葡聚糖酶吸附率/β-葡萄糖苷酶吸附率2.21。 采用“底物原位吸附-固液分离”拆分纤维素酶系中的β-葡萄糖苷酶，用该酶定向水解低聚木糖生产废渣制备纤维低聚糖，与未拆分纤维素酶相比，纤维低聚糖得率从21.26%提高到23.76%水解产物中纤维低聚糖占总糖的百分率从42.01%提高到66.97%葡萄糖、木糖、纤维二糖和纤维低聚糖增殖嗜酸乳杆菌的研究结果表明，纤维二糖的增殖效果最好，增殖了13.26倍，葡萄糖其次，增殖了10.71倍，纤维低聚糖增殖了9.07倍，嗜酸乳杆菌不能代谢木糖。 关键词：纤维低聚糖；低β-葡萄糖苷酶纤维素酶；嗜酸乳杆菌  Abstract The main purpose of this research aims at separating the β-glucosidase,of the cellulose enzymes system with institute adsorption of substrate - liquid separation,and on the oligosaccharide residue of cellulase production and the adsorption conditions were optimized,somehow with the enzyme hydrolysis of xylo-oligosaccharides prepared by directional fiber production oligosaccharide residue; by in vitro methods of anaerobic culture of Lactobacillus acidophilus metabolism of glucose, xylose, cellobiose, fiber oligosaccharides law and metabolites were analyzed. The main results are as follows: Studies on the temperature, pH, adsorption time on the oligosaccharide residue of cellulase production and the impact of each component ,optimal conditions of adsorption temperature in 10 ℃, pH7.0, 30min which,demonstrates that endoglucanase adsorption rate are 87.67%, β-glucosidase adsorption rate are 39.74%, the adsorption rate of endoglucanase / β-glucosidase adsorption rate remains 2.21. Using in situ adsorption of substrate - liquid separation, separation of cellulose enzymes in the β-glucosidase oligosaccharide hydrolysis by enzyme production directed preparation of fiber residues of oligosaccharides, and not split cellulose compared to the yield of oligosaccharides from the fiber 21.26% Up to 23.76%， Hydrolysis products of the per