恒河猴脊髓半断模型行脑和脊髓fmri检查麻醉方案的试验研究.docVIP

恒河猴脊髓半断模型行脑和脊髓fMRI检查麻醉方案的实验研究 田肇隆1 , 王小华1, 许亚超1 , 李晓光2 , 饶家声 2, 赵璨2 首都医科大学宣武医院麻醉科 北京市 100053 2.北京航空航天大学 北京市 100191）摘要目的 为需要进行核磁共振扫描的恒河猴及其他大型实验动物提供一种简捷、迅速、安全的麻醉方法。方法 采用军事医学科学院实验用恒河猴7只，均为雌性，体重4~7公斤，采用基础+持续静脉麻醉（氯胺酮+丙泊酚）基础麻醉应用氯胺酮10mg/kg,im,待猴入睡后开放静脉建立持续静脉通道，三通连接丙泊酚200mg/100ml0.9%氯化钠注射液,丙泊酚以0.3mg/kg/min持续输注,氯胺酮1mg/kg/30min间断给予。fMRI中将恒河猴置于特制的实验架上并将门齿托起以保持呼吸道通畅，试验中监测呼吸、心率、角膜反射及瞳孔状态。结果 共进行实验44次，未发生动物躁动、呼吸抑制、心律失常、动物死亡等严重并发症，实验中恒河猴心率、呼吸维持平稳，整个实验顺利完成。实验结束停药后，实验动物很快清醒，并安全返回。结论 在较长时间的核磁共振恒河猴实验中氯胺酮基础麻醉+丙泊酚持续静脉麻醉是一种全新的安全、便捷，实用的麻醉方式。 关键词恒河猴功能磁共振全凭静脉麻醉[文献标识码] A [文章编号] Doi: 10. 3969. j. issn. 1671. 7856. 2013.000.000 Experimental study of continuous intravenous anesthesia for fMRI of Rhesus monkey TIAN Zhao-long 1; WANG Xiao-hua 2; XU Ya-chao1; LI Xiao-guang 2; RAO Jia-sheng 2; ZHAO Can 2; (1.Xuanwu hospital Capital Medical University, department of anesthesiology, Beijing 100053, China 2. Beijing University of Aeronautice Astronautics, Beijing 100191, China) [Abstract] Objective fMRI animal experiments that require sustained anesthesia to provide a simple, convenient, and safe manipulation condition. This investigation was designed to confirm the appropriate anesthetic regimen for fMRI experiment of rhesus. Method Seven rhesus from Academy of Military Medical Sciences are female, weight 4~7 kg using continuous intravenous anesthesia (ketamine + propofol) . Ketamine 10mg/kg im until the rhesus sleep then establish a continuous intravenous access. continuous injected propofol 0.3mg / kg / min, then intermittent ketamine 1mg/kg/30min. The rhesus placed in a special experiment rack and the incisor hold up to keep the airway open. During the whole test we monitored breathing, heart rate, corneal reflex and pupil. Results 44 times experiments successfully completed, no animal have restlessness, respiratory depression, arrhythmia, deaths and other serious complications. At the end of the experiment, the experimental animals awake soon. Conclusion continuous intravenous anesthetic o