盐胁迫下小麦叶片蛋白的双向电泳及图谱分析-中国农学通报.PDF

中国农学通报 2013,29(12):44-48 Chinese Agricultural Science Bulletin 盐胁迫下小麦叶片蛋白的双向电泳及图谱分析 牛银银，张二朋，张 硕，梁笑辉，方显明，黄象男，杨冬之 （郑州大学生物工程系，郑州450001 ） 摘 要：为了建立盐胁迫条件下小麦叶片蛋白的双向电泳研究方法，进一步获取小麦叶片响应盐胁迫的 差异蛋白图谱，以河南省主导小麦品种‘周麦18’为实验材料，利用NaCl 溶液模拟盐分胁迫，在建立小麦 幼苗双向电泳技术和图像分析体系的基础上，对盐胁迫下的小麦幼苗进行差异蛋白组学初步分析。结 果表明：72 h 1.0% NaCl 溶液胁迫下，小麦幼苗蛋白表达组发生了变化；其中共有332 个点得到匹配，获 得61 个差异表达的蛋白点，29 个表达量显著上调，32 个显著下调。这表明，在盐胁迫环境条件下，植物 为了满足对NaCl 的需求，可能开启和关闭了体内的一些相关代谢途径。 关键词：小麦；盐胁迫；蛋白质组学；双向电泳；差异蛋白表达 中图分类号：Q25 文献标志码：A 论文编号：2012-3621 The Analysis of Two-dimensional Electrophoresis Maps for Wheat Leaf Protein Under Salt Stress Niu Yinyin, Zhang Erpeng, Zhang Shuo, Liang Xiaohui, Fang Xianming, Huang Xiangnan, Yang Dongzhi (BioengineeringDepartment,ZhengzhouUniversity, Zhengzhou 450001) Abstract: The aims were to set up an approach on two-dimensional electrophoresis for wheat leaf proteins under salt stress, and obtain the discrepancy-expressed protein map responded to high-salt content in soil. For this study, the experimental material was a main wheat cultivar ‘Zhoumai- 18’in Henan, NaCl solution was utilized to imitate the salt stress. Based on the establishment of two dimensional electrophoresis and image analysis, the proteomic difference of wheat seedlings under salt stress was initially analyzed. The results showed that, the whole proteins extracted from young seedlings cultured in pots under 1.0% NaCl for 72 hours were changed. 332 proteins were matched and 61 proteins showed differently expressed under salt stress, with 29 up and 32 down. This indicated plants maybe turn some relevant metabolic pathways inside them on and off under salt stress in order to satisfy its need for NaCl. Key words: wheat; salt