应用干细胞和自制胶原-壳聚糖构建组织工程心瓣膜-第三军医大学学报.DOCVIP

应用骨髓干细胞和自制胶原-壳聚糖构建组织工程心瓣膜 付建华1，2，赵 曼3，杜桂英4，杨达宽2，黄云超5（252000 山东 聊城，聊城市人民医院：胸心外科1，信息科3；650031 昆明，昆明医学院：第二附属医院胸心外科2，第三附属医院胸心外科5；252000山东 聊城，聊城市疾控中心艾防所4） [作者简介] 付建华，电话：（0635）8991028，E-mail： jianhuafu1120@163.com [摘要] 目的 探讨应用骨髓基质干细胞（bone mesenchymal stem cells ，BMSCs）诱导分化的内皮细胞为种子细胞，以胶原-壳聚糖复合物为支架材料，运用组织工程学的原理和方法体外构建组织工程心脏瓣膜（tissue engineering heart valve,TEHV）的可行性。方法 兔主动脉壁体外分离、培养、传代平滑肌细胞、成纤维细胞，将培养的成纤维细胞、平滑肌细胞与兔骨髓基质干细胞诱导分化的内皮细胞按顺序种植在预湿处理的胶原-壳聚糖复合支架上，通过组织学及倒置显微镜、扫描电镜观察内皮细胞、成纤维细胞、平滑肌细胞在支架上生长、增殖和基质分泌情况。结果 HE染色示内皮细胞能够在胶原-壳聚糖复合支架粘附、伸展、增殖，在TEHV表面长成连续的细胞层；扫描电子显微镜;内皮细胞 [中图法分类号] [文献标志码] A The primary experimental stuy of constructive tissue engineering heart valve using BMSCs and self-making collage-chitosan complex scaffold Fu Jianhua1,2, Zhao Man3, Du Guiying4, Yang Dakuan2, Huang Yunchao5 (252000 Shandong Liaocheng ,Liaocheng People’s Hospital：Department of Cardiac and Thoracic Surgery1, Department of Information3;650031 Kunming, Kunming Medical College ;Department of Cardiac and Thoracic Surgery of the Second Affiliated Hospital2, Department of Cardiac and Thoracic Surgery of the Third Affiliated Hospital5; Instituit of AIDS Prevention and Therapy4, Liaocheng ,252000 ,Shandong,China) [Abstract] Objective To explore feasibility of constructing TEHV using endothelial cell induced and differentiated by BMSCs as seeded cells and collagen-chitosan complex as scaffold materials, applying theory and method of tissue engineering .Method Smooth muscle cell , fibroblast cultured by rabbit aortic wall, Fibroblast, smooth muscle cells and endothelial cell induced and differentiated by BMSCs seed in pre-moisture collagen-chitosan complex scaffold in turn , observing cells to adhere to, grow ,proliferate and secrete stroma , using histochemistry , inverted microscopy , SEM scanning observation .Result endothelial cells can adhere to, grow and proliferate on collagen-chitiosan complex scaffold by HE staining observation , cells on TEHV surface grow in a serial c