基于微型光谱仪干化学法检测电解质的实验研究.docVIP

基于微型光谱仪干化学法检测电解质的实验研究 白 晓，李 强，吴杰红，蒲晓允，李 锋，傅灵媛，项贵民 摘要：目的 研究一种基于微型光谱仪和干化学试剂技术快速检测电解质的方法。方法 将配制的钾、钠、氯液体试剂用不同的程序冻干于检测杯中，封存。加入标本和稀释液后，经磁力搅拌、加热6min后,用微型光谱仪检测光密度值。结果 各指标线性范围、准确性及精密度均基本达到检测要求，抗干扰性能良好。各指标测定结果与干化学分析仪VITRO S-250具有良好的相关性，其r≥0.98。结论 以微型光谱仪干试剂法测定电解质结果准确可靠，检测过程简便，携带方便，适于战地救治、基层医疗的需要。 关键词：微型光谱仪；干试剂；电解质 中图法分类号： 文献标识码： Experimental Study on the serum electrolyte determination based on the Minispectromete with dry reagent BAI Xiao, LI Qiang, WU Jie-hong , PU Xiao-yun ,et al. (Department of laboratory medicine, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China) Abstract: Objective To establish a new assay for the rapid determination of serum electrolyte based on the Minispectromete with dry reagent. Methods The reagent of the electrolyte was dried in determination-well by the different program and was sealed with smoothy plastic film before use. It was detected by the Minispectromete, after it was magnetic agitated and incubated in the 37℃ within 6 minutes. Results The linear range can meet the demand for clinical analysis. The intraassay coefficients of variation was less than 4%, and interassay coefficients of variation was less than 5%. The average recovery was 95%-105%. There was a good correlation between the results and that of the VITROS-250. Hb concentration 11.50g/L,TG concentration 11.20 mmol/L and bilirubin leve 290.40umol/L no interference was observed. Conclusion The method was accur, reliability and convenience, which may contribute to military laboratory medicine. Key words：Minispectromete; dry reagent; lectrolyte *目前, 对于电解质的测定，大多数实验室采用离子选择性电极(ISE)法进行检测。由于电极、试剂费用较高,已有很多实验室逐步倾向使用酶法,且评价较好[1,2]。电解质K+、Na+、Cl-的测定是临床诊治和抢救的常规及急诊检测项目,具有重要的临床意义，也是军事医学中战地救治研究的重要内容之一。而目前，能适于野战环境的便携式急救生化仪器非常少[3]。我们与重庆大学合作，研制适合野战、基层等小型医疗机构的便携式检测设备。在微型光谱分析仪的基础上，对光信号采集、处理、分析软件进行了优化，通过对干化学试剂的研制,使整个装置体积减小、提高其检测灵敏度，操作简便，并便于储存和运输。本文将目前的研究情况作简要汇报。 1 材料与方法 1.1 仪器 微型光谱分析仪（重庆大学研制），波长范围在360～780nm，吸光度线性测定范围为0.000～3.000；ModulyoD-230冷冻干燥机（美国Therm