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真菌形态的几种观察方法(The observation method of several fungi morphology)
真菌形态的几种观察方法(The observation method of several fungi morphology)
The observation method of several fungi morphology
Fungi as an experimental material, used in the microbiology laboratory is quite common, the correct observation method can accurately reflect the culture and its essence. Here are some form of fungi in the laboratory observation method.
The 1. slide culture method
Slide culture method is a basic method of laboratory observation fungal morphology. The basic steps of this method is: under sterile conditions, with sterile Straw from solid culture medium for fungi to melt quickly, a drop in sterile glass slides after cooling, by inoculating loop picks up a little or a little section of mycelium spores medium on the solidification on top a sterile coverslip. Then, this film will dish into a clean, absorbent filter bottom of culture dish into the moist, or will the dish put some water, the middle with a glass rod uplift, will do a good job of training a film on top, suitable temperature culture. The cultivation of a good film removed with tweezers gently remove the top coverslip, put onto a clean glass slide, to be dry, on both sides with a transparent plastic fixed, dyed with cotton blue staining.
Slide culture method can also be that: the plate thickness of 0.5mm medium with a knife cut the size of 0.5cm2 is small, a small medium were placed on clean slides, inoculation, the same culture observation method. Can also be a good culture medium contained excess on the glass cutting blade for dry, use a larger coverslip covered hyphae, fixed, staining with the same method, morphological observation on both sides, this method is particularly applicable to produce spores of fungi. This method should pay attention to the following points: medium can not drop too much, the inoculum is not too much to prevent pollution.
2. insert cultivation method
Insert cultivation method is a basic method of laboratory observation of fungal morphology. The basic steps of this meth
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