.LightCycler480系统特点及应用方向.ppt

7500第一提出定量快速的概念，随后epp公司06年推出银质模块的快速定量PCR仪，BIORAD08年推出CFX96快速定量PCR仪 7500第一提出定量快速的概念，随后epp公司06年推出银质模块的快速定量PCR仪，BIORAD08年推出CFX96快速定量PCR仪 每个厂家都知道边缘效应，以及它的危害，也都在采取各种方法来降低或消除边缘效应的影响。 Serial 1:10 dilutions with 9 replicates of a plasmid DNA sequence were amplified with the LightCycler? 480 Probes Master and detected with a Universal ProbeLibrary probe. The PCR result shows a log-linear relationship over a broad dynamic range (10 log-intervals) and high reproducibility (Cp level) for replicates of the different dilution steps. Plasmid DNA, 20μl reaction. One amplicon containing two SNPs next to each other (neighboring bases in same codon); one probe pair is able to distinguish all combinations that occur in this sample set (note that double mutants with codon TAG were not present). Targte: Apolipoprotein B Detection with Hybridization Probes at 640 nm The LightCycler? 480 thermal block cycler is available for either 96- or 384-well plates; there are two catalog numbers for the instrument, depending on the block you need. Once installed, the block cycler is easily accessible and can be interchanged easily within minutes by users - without special training. This change also includes the block cycler cover (heated lied with pinholes). There is no requirement for an experienced service technician to do this. Each block cycler version is also available separately and comes in a specially designed loading help box. The upper part of the box is first placed next to the opened instrument; it can then accept the first block. The new block is inserted and the empty lower part of the box then used as the cover for the block that has just been removed. After changing block cyclers, the experiment can be continued without any time-consuming recalibration. The instrument automatically recognizes the new block cycler. In combination with the Roche LightCycler? 480 System, instead of taking 6 hours or even a complete working day, you now only need a total workaround time from sam