肥胖哮喘动物模型的建立.docVIP

肥胖哮喘动物模型的建立 夏宇，黄英袁红欣王模奎(重庆医科大学附属儿童医院呼吸科 400014) 摘要：目的：探索在高脂饲料诱导肥胖基础上建立哮喘动物模型，并对模型进行评估。方法：40只3周断乳C57BL小鼠随机分为4组，每组10只。分别进行普通饲料喂养+NS致敏激发（A组）；普通饲料喂养+OVA致敏激发（B组）；高脂饲料喂养+NS致敏激发（C组）；高脂饲料喂养+OVA致敏激发（D组），比较各组。结果：C、D组体长、L’s指数、肝脏、肝脏/体均明显大于A、B组（P﹤0.05）②A、C组小鼠肺组织无炎症，B、D组炎症明显，D组更甚③支气管肺泡灌洗液细胞总数、嗜酸性粒细胞比例D组明显高B组明显高于A组（P﹤0.05）④B、D组肺泡灌洗液中IL-4分别较A、C组明显升高（P﹤0.05），IFN-γ则明显降低（P﹤0.05），D组IL-4/IFN-γ明显高于B组。C、D组各只小鼠未达肥胖标准，B、D组小鼠各死亡只,均淘汰出组；结论：OVA致敏、激发C57BL小鼠成功建立了肥胖哮喘模型。 中图法分类号：R-332 Establishment of mouse obesity asthma model XIA Yu，HUANG Ying，YUAN Hong-xin, WANG Mo-kui, LOU Xiao-liDepartment of Respiratory Diseases, Children’s Hospital, Chongqing Medical University, Chongqing 400014, China) Abstract Objective To establish an obesity animal model in mice and evaluate the model. Methods A total of 40 clean 3-week-old weaning female C57BL/6J mice were randomly and equally divided into 4 groups. Group A were fed with normal diet food and sensitized with normal saline (NS), group B were fed with normal diet and challenged with ovalbumin (OVA). Groups C and D were fed with high fat diet and only the later received OVA challenging. Body weight, liver weight, index of liver/body weight, Lee’s index, lung morphology, cell number of bronchoalveolar lavage fluid, concentration of IL-4 and IFN-γ were measured or calculated. Results (1) The body weight, liver weight, index of liver/body weight, Lee’s index of group C and D were significantly higher than those of group A and B. (2) Local inflammation was significant in groups B and D, with the later more serious. (3) Total cell count and ratio of eosinophil of bronchoalveolar lavage fluid were highest in group D followed by group B, and then group A. (4) Groups B and D had a higher concentration of IL-4 and lower level of IFN-γ in comparison with group A and C, and the ratio of IL-4/ IFN-γ was higher in group D than group B. (5) Two mice respectively from groups C and D had not meet the obesity criterion before challenge, and the other 2 mice from group B a