pcr扩增过程中常见的问题及解决方法（Common problems and solutions in the process of PCR amplification）.docVIP

pcr扩增过程中常见的问题及解决方法（Common problems and solutions in the process of PCR amplification） Common problems and solutions in the process of PCR amplification PCR technology is highly specific, selective and sensitive, and it is fast, simple and easy to automate. Therefore, it has received extensive attention and reasonable application in clinical medical work. At present, many primary medical institutions in China have carried out the application work of PCR research. PCR technology is very simple, the principle is very clear, so the conditional units can be successfully launched, but after all, PCR technology is a new thing, is influenced by many factors, so it is not easy to do well. In the application of PCR technology, it will encounter such or such problems, and even get the opposite result of the fact. In order to enable us to successfully carry out PCR work, better play the role of the tool of PCR technology, we summed up the work of the PCR according to the experience of many years, the paper summarizes some of the problems often occur during PCR amplification, available to the majority of researchers as a reference, welcome to correct deficiencies. False positive amplification In the course of the experiment will sometimes encounter inspection samples were all positive, and the amplified product bands of brightness uniformity, this is a manifestation of amplification system pollution most typical, need to check carefully, eliminate sources of pollution, in general from the following steps: The pollution of reagent: The reagents or components provided by the factory are contaminated in the process of leaving the factory or transportation or storage. Test method, according to the reagent box instructions, the reagent will be packaged, and directly placed in the PCR instrument amplification (without negative contrast, plus a little amount of distilled water), you can simply and effectively judge whether the reagent has been contaminated. The laboratory of pollution: