大豆根际土壤镰孢菌不同分离方法比较.pdfVIP

第27卷 第1期 大 豆 科 学 Vo1．27 N仉l 2008拒 2月 SOYBEAN SCIENCE Feb． 2o08 Comparing Different Methods for Isolating Fusarium from Soybean Rhizo- sphere Soil LIU Jin．bo ，一，XU Yan．1i ，and WEI Wei ， ， ( Northeast Institute of Geography and Agricultural Ecology，CAS，Harbin 150081，Heilongjiang； Graduate School，CAS，Beijing 100049；’Northesat Fomstry University，The college of life science，Harbin 150040，Heilongjiang，China) Abstract：Fusarium root rot of soybean is one of the soil．borne diseases which is dif~cult to eontro1．In soil，distribution of Fusarium is surprisingly diverse，which can efficiently influence the pathogenic Fusarium populations and host resistance．I· solation of Fusarium is very useful in the study of soil Fusarium diversity．Many methods for the isolation of Fusarium have been reported，but the efficiency was certainly different．In this paper，some related contents which affect the effeeieney of Fusarium separation were researched．and dilution plate method and direct soil plating method were compared．The results showed that bacteria could be inhibited exeeUenfly when the concentration of antibiotic reached fourfold，but there was no significant impact on the number offungi．Interestingly，it seems that different combination of antibiotics had effect on Fusar— ium isolation．Between colony forming units(CFU)of fungi and different sample volume，well hnear relationship were de· tected in both sterile water and Water Agar treatment(R 0．9)．The isolation rate of Fusarium in sterile water treatment was 21．O％ ，a bit higher than that in Water Agar treatment，which was 10．O％．No significant effects on Fusarium isolation between two culture media were observed．Compared with Malachite Green Agar(MGA)，Peptone PCNB Agar(PPA)be． hayed h