含叶酸功能端基Pluronic F127的合成及其在水中的形态.pdfVIP

2008年第66卷 化 学 学 报 Vo1．66．2008 第1期．138～140 ACTA CHIMICA SINICA No．1．138～140 · 研究简报· 含叶酸功能端基Pluronic F1 27的合成及其在水中的形态 邵芳可“ 吴 唯“ 查刘生易 张 琰 a超细材料制备与应用教育部重点实验室 华东理工大学材料科学与工程学院 上海 200237) ( 东华大学分析测试中心 上海 200051) 摘要 将三嵌段共聚物Pluronic F127依次与对甲苯磺酰氯、邻苯二甲酰亚胺化钾及乙二胺反应，使端羟基转化为端氨 基，然后与具有生物活性的大分子叶酸反应，使其作为靶向配体接到F127的端基上．利用 HNMR和IR对叶酸封端的 F127嵌段共聚物(F127-folate)结构进行了表征．用透析法制备胶束溶液，利用TEM研究了F127．folate在水溶液中的 形态，结果表明F127．folate在水溶液中形成了胶束结构． 关键词 Pluronic F127；叶酸；胶束 Synthesis of Folic Acid Conjugated Pluronic F1 27 and Their Morphology in Water SHAO，Fang—Ke“ WU，Wei“ ZHA，Liu—Sheng ZHANG，Yan aKeyLaboratoryforUltrafineMaterialsofMinistryofEducation，SchoolofMaterialsScienceandEngineering，EastChina University ofScience and Technology，Shanghai 200237) ( Research Centerfor Analysis and Measurement，Donghua University，Shanghai 200051) Abstract Triblock copo1) mer Pluronic F127 was reacted with P—toluenesulfonyl chloride，potassium phthalimide and ethylenediamine in turn to modify the end hydroxyls of F127 to amino end groups．Using the amino end groups，the bioactive macromolecular folic acids were conjugated to the end group of F127 as the targeting ligand． H NMR and IR were adopted to characterize the structures of the folate—capped F127 copolymers．rrI1e micelle solutions of F127一folate were prepared by the dialysis method．rrI1e morphologies of the copolymers in water were investigated by TEM．rrI1e results proved that F127一folate could self-assemble into micelles in water． Keywords Pluronic F127；folic acid；micelle 聚