基质金属蛋白酶-9表达与胃癌侵袭和转移的关系.pdfVIP

中尉医 装备2【)()8年2月第5卷第2期 China Medical Equipment 2008 February Vo1 ． 5 NO．2 基质金属蛋白酶一9表达与胃癌侵袭和转移的关系 周兆海① [文章编号】1 672—8270(2008)02—0052—02 [中图分类号】R 573 [文献标识码】B 【摘要】目的：观察胃癌组织中基质金属蛋白酶9(mmp一9)的表达与侵袭程度和淋巴结转移的关系。方法： 应用免疫组化s—P法检测67例胃癌组织中mmp一9的表达。结果：mmp-9表达强度与早期胃癌及进展期胃癌 的病理分期显著相关 (PO．O1)，与淋巴结有无转移显著相关(尸0．Ol o结论：ppm一9表达强度与胃癌 分期以及有无淋巴结转移有显著相关性，是判断胃癌分期和转移有价值的指标。 [关键词】基质金属蛋白酶9 胃癌 免疫组织化学 The orrelation between the expression of matrix metalloproteinase(MMP．9)and degree of invasion and lymph node metastasis／Zhou Zhaohai／／China Medical Equipment，2008，5(2)：52-S3 [Abstract]Objective：To investigate the correlation between the expression of matrix metalloproteinase(MMP-9) and degree of invasion and lymph node metastasis．Method：S．P immunohistochemical method was used to detect gastric cancer tissue MMp-9 expression in 67 cases．Resuit：There was significant correlation between MMp-9 expression degree and pathological staging of early stage gastric cancer and progressive gastric cancer(尸0．0 1)，as well as lymph node metastasis(尸0．01)．Conclusion：There was significant correlation between MMP-9 expression degree and gastric cancer staging and lymph node metastasis，MMP-9 expression degree is a valuable index for gastric cancer staging and metastasis judgement． [Key words]Matrix metalloproteinase一9，Gastric cancer,Immunohistochemistry [First·author’S address]The first people’S hospital of MuDanJiang，HeiLong]iang 15701 1，China 肿瘤的侵袭和转移是多步骤、多阶段的复杂 所有蜡块行5 um连续切片各3次，做HE~E]免疫 过程，而首先突破基底膜是肿瘤细胞侵袭转移的 组化染色。磷酸盐缓冲液体带I抗体作阴性对照。 最初和关键阶段。今年来研究表明，基质金属蛋 免疫组化试剂：福州迈新生物技术开发公司，采用 白酶 (matrix metaUoproteinases mmps)通过 s-p法，染色步骤安说明进行。以细胞浆染成棕黄 细胞外基质 (extracell matrix ecm)降解促进 色为阳性细胞。高倍镜下取3个不同视野各计数l00 癌细胞对周围组织的侵袭和转移…。其中基质金属 个细胞。阳性细胞在1％～30％为低表达，大于30％ 蛋白酶-9(mmp一9)在肿瘤细胞突破基质膜屏障中 为高表达