基于LC―MS技术毛细支气管炎风热闭肺证的代谢组学研究.docVIP

基于LC―MS技术毛细支气管炎风热闭肺证的代谢组学研究 　　摘要：目的 采用高效液相色谱-质谱联用技术的代谢组学方法，探析毛细支气管炎中医风热闭肺证在血清内源性代谢物的变化，以寻找毛细支气管炎中医风热闭肺证的诊断标志物。方法 收集毛细支气管炎患儿风热闭肺证（风热闭肺组）20例和健康儿童（对照组）20例。以液相色谱-质谱联用技术对两组受试患儿的血清样本进行代谢图谱分析，并对潜在的生物标记物进行鉴定。结果 风热闭肺组与正常对照组相比很多离子峰高、峰面积均存在着明显差异，筛出具有统计学差异的差异生物标志物共8种：L-缬氨酸，尿素，L-天门冬氨酸，2氨-咪唑，D-甘露糖，L-天门冬酰胺，D-果糖，L-谷氨酰胺。结论 本研究从代谢组学小分子水平初步反映了小儿毛细支气管炎风热闭肺证的代谢特征，为从生物代谢的角度研究中医证候的现代科学内涵提供了一定的实验依据。 　　关键词：代谢组学；毛细支气管炎；风热闭肺证；液相色谱-质谱联用技术 　　Study on Metabonomics of Bronchiolitis with Wind Heat Obstructing Lung Syndrome Based on LC-MS 　　CHEN Chao，BIAN Xun 　　（Department of Pediatrics，Nanjing Traditional Chinese Medicine and Western Medicine Hospital，Nanjing University of Traditional Chinese Medicine，Nanjing 210014，Jiangsu，China） 　　Abstract：Objective Using high performance liquid chromatography-mass spectrometry metabonomic technique study method，the change of capillary bronchitis chinese wind heat closed the lung in serum endogenous metabolites，to find the diagnosis of bronchiolitis in Traditional Chinese Medicine of wind heat closed lungs markers.Methods Capillary bronchitis in children with wind heat closed the lung（wind heat closed lung group）and 20 healthy children（control group）20 cases.The two groups of serum sample test were analyzed by using metabolic profiling by liquid chromatography mass spectrometry，and to identify potential biomarkers.Results Many group of wind heat lung ion peak high compared with the normal control group，area there were significant differences， with statistical difference between screening biomarkers were 8： L-valine， urea， L-aspartic acid， 2 of ammonia - imidazole，D-mannose，L-asparagine，D-fructose，L-glutamine.Conclusion This study from the metabonomics small molecular level preliminary reflect the metabolic characteristics of children with bronchiolitis in wind heat closed the lung，and provide a certain experimental basis for the modern scientific connotation of TCM syndromes from the perspective of biological metabolism. 　　Key words：Metabonomics；Bronchiol