免疫检测技术在中药质量快速评价中的应用.docVIP

免疫检测技术在中药质量快速评价中的应用 　　[摘要]中药质量评价分为有效性和安全性2个方面，现有的中药质量评价方法以仪器检测方法居多，不能满足生产及生活中简单、快速、?F场化的实际需求。免疫检测技术具有灵敏度高、特异性强、操作简单、检测快速、检测成本低、仪器依赖性低、易于现场化等优点，在临床检验、食品安全检测及环境污染监测等领域应用广泛。近些年来，免疫检测技术逐渐应用于中药质量控制领域，涉及中药有效成分的定量检测，中药有害物质检测，中药外源性污染物检测等方面。该文综述了应用较广的酶联免疫检测法和胶体金免疫检测试纸条技术的原理及在中药质量评价中的应用，该技术在中药质量现场快速检测中具有较好应用前景。 　　[关键词]质量评价； 快速检测； 酶联免疫检测； 胶体金免疫检测试纸条 　　[Abstract]The quality evaluation of traditional Chinese medicine can be divided into two aspects， effectiveness and safety. The existing methods for evaluating the quality of traditional Chinese medicine（TCM） are mostly based on the testing instruments， which can not meet the practical needs of simple， rapid and on-site in production and life. Immunoassay， characterized by simple， rapid， sensitive， specific， low-cost and high-throughput， is widely used in the fields of clinical diagnosis， environmental pollution monitoring， food safety testing， and other fields. In recent years， immunoassay technology has been gradually applied in the field of quality control of TCM， involving quantitative detection of effective components of TCM， detection of harmful substances in TCM， and detection of exogenous pollutants in TCM. This paper summarizes the principle of the wide application of ELISA and colloidal gold immunostrip technology and its application in quality evaluation of TCM， this technique has a good application prospect in the field of rapid detection of the quality of TCM. In this paper， the principles of the widely used ELISA and colloidal gold immune test strip technology as well as their application in quality evaluation of TCM were reviewed， and the results showed that this technique had a good application prospect in the field of rapid detection of the quality of TCM. 　　[Key words]quality evaluation； fast detection； ELISA； colloidal gold immune test strip 　　中药有效性和安全性是中药质量评价的2个重要标准。化学成分是中药发挥疗效的物质基础，其中又以指标性成分的含量高低作为中药质量控制及品质优劣评价的重要指标。近些年来，随着中药野生资源的大量减少，及基于对中药质量稳定性和可控性的需求，中药栽培品逐渐成为中药市场的主要来源。在中药的栽培管理过程中，农药的大量使用导致农残偏高，加上栽培环境