CyclinD1及P21在肥大乳房腺体组织中表达及意义.docVIP

CyclinD1及P21在肥大乳房腺体组织中表达及意义[摘要]目的：探讨肥大乳房中CyclinD1和P21的表达级意义。方法：采用免疫组化SP法，分别测定了34例腺性肥大乳房和30例正常体积乳房腺体中CyclinD1和P21的表达。结果：CyclinD1在34例肥大乳房中，阳性18例，阳性率52.94%；30例正常对照组中，全部表达阴性，阳性率0，两组间比较具有统计学意义（P＜0.05）。P21在34例肥大乳房中，3例阳性，阳性率8.82%；正常对照组30例，阳性9例，阳性率30.00%。两组间比较具有统计学意义（P＜0.05）。结论：乳房肥大的形成可能与CyclinD1增多和P21减少有关。 [关键词]CyclinD1;P21;乳房肥大 [中图分类号]R730.45[文献标识码]A[文章编号]1008-6455（2011）10-1556-02 Expression of CyclinD1 and P21 in the mammary gland of the hypertrophic breast and their significance JIN Jun-hua,ZHANG Rui-rui,WANG Qi,XIE Qun,LIU Dan-dan,HUI Zu-yan,HU Qiang (Department of Plastic Surgery,the Central Hospital of Xiangyang,Xiangyang 441021,Hubei,China) AbstractObjectiveTo detect the expression of CyclinD1 and P21 in the mammary gland of the hypertrophic breast and the normal voluminal breast,so that their function and significance in the pathogenesis of breast hypertrophy can be investigated.MethodsThe breast tissue samples were obtained, including hypertrophic breast tissue(34 cases) and normal voluminal breast tissue(30 cases).They were detected by the SP method immunohistochemistry for the expression of CyclinD1 and P21.ResultsThe positive expression rate of CyclinD1 in the hypertrophic breast was 52.94％(18 of 34),while 0 in the normal voluminal breast(0 of 30),showing a significant difference between the two groups（P P21是细胞周期蛋白和细胞周期蛋白依赖性激酶的广泛抑制剂，是野生型P53基因下游靶基因的转录产物[3-4]，可与多种Cyclins/CDK复合物结合，抑制底物磷酸化，使细胞周期停滞于G1期，进而抑制细胞增殖。另外，P21还能抑制细胞增殖核抗原（PCNA）依赖性DNA复制，通过直接抑制DNA合成来抑制细胞增殖[5]。本研究中,与正常体积乳房组相比，肥大乳房组中P21表达降低，两组间比较差异有统计学意义（P＜0.05）。提示可能P21的减少对Cyclins/CDK的抑制作用降低也参与了乳房增生肥大。 过去对于CyclinD1和P21的研究多集中在肿瘤方面，两者之间的动态平衡才可以保持细胞的正常增殖，若两者之间的平衡被打乱，则会导致细胞周期紊乱，致使细胞异常增生。本课题采用免疫组化的方法检测了34例肥大乳房和30例正常体积乳房，发现较正常体积乳房，肥大乳房中存在CyclinD1的增多和P21的减少，提示这两个因子之间的表达失衡可能与乳房肥大的发生密切相关。 [参考文献] [1]Dancey A,Khan M,Dawson J,et al. Gigantomastia-a classification and review of the literature[J]. Plast Reconstr Aesthetic Surg,2008,61(5):493. [2]张兆祥,孙家明.表皮生长因子在肥大乳房腺体组织中的表达及意义[J].中国美容医学杂