复方红景天制剂对高+Gz应激大鼠血清皮子酮及-中国试验动物学报.DOC

复方红景天制剂对高+Gz应激大鼠血清皮质酮及心肌组织 糖皮质激素受体的影响 陈良恩，詹 皓，吴 峰，赵安东，杨明浩 （空军航空医学研究所，北京 100142） 【摘要】目的 研究复方红景天制剂只雄性大鼠随机分为组，对照组、+10Gz应激组低、中高剂量组，每组12只。各组均灌胃给药14 d，第15 进行+10Gz离心机暴露，各组动物各组动物。+10Gz应激组+10Gz应激大鼠血清皮质酮浓度，上调心肌组织糖皮质激素受体表达水平，药物中、高剂量组大鼠血清皮质酮浓度明显低于+10Gz应激组+10Gz应激组致心肌损伤Effects of rhodiola crenulata compound on serum corticosterone and myocardial glucocorticoid receptor in rats exposed high sustained +Gz Chen Liang-en，Zhan Hao，Wu Feng，Zhao An-dong，YANG Ming-hao （Institute of Aviation Medicine, Air Force, Beijing 100142,China） 【Abstract】 Objective To study the effect of rhodiola crenulata compound on serum corticosterone and myocardial glucocorticoid receptor(GR) in rats exposed high sustained +Gz. Methods Seventy-two healthy SD rats were randomly divided into six groups: blank control group, stress control group, +10Gz stress group and low, medium, high dose drug group, with twelve rats in each. 20ml/kg menstruum was fed to each rat once per day for 14 days. Low medium, high dose drug group were fed with rhodiola crenulata compound at doses of 0.75g/kg, 1.5g/kg and 3.0g/kg respectively, the other three groups were fed with equal volume saline. Rats were exposed to high +Gz in 15th day. The concentration of corticosterone in the serum of each group rats was detected with ELISA. Western blot was used to detect the expressions of GR in the myocardium of each group rats. Results The content of corticosterone was significantly elevated in +10Gz stress group, while the expression of GR in the myocardium was markedly declined (P0.01, P0.05). However, compound preconditioning could decrease concentration of corticosterone in the serum and enhance the expression of GR in the myocardium from rats after ＋10Gz exposure. The corticosterone concentration of medium and high dose drug groups was significantly lower and the level of GR expression in the myocardium was significantly higher than that of +10Gz stress group (P0.05, P0.01). Conclusion Rhodiola crenulata compound preconditioning co