可溶性Jagged1-Fc嵌合蛋白对小鼠淋巴细胞活化、增殖和周期的影响.docVIP

　　可溶性Jagged 1/Fc嵌合蛋白对小鼠淋巴细胞活化、 增殖和周期的影响 ：邢飞跃, 于哲, 刘静, 季煜华 【摘要】 　　目的: 研究可溶性Jagged 1/Fc嵌合蛋白在小鼠淋巴细胞活化、 增殖和周期中的作用。方法: 选择有效剂量为500 μg/L Jagged 1/Fc嵌合蛋白（Jagged 1/Fc）, 以活体染料/羧基荧光素乙酰乙酸琥珀酰亚胺酯染色, 建立在多克隆刺激剂刀豆蛋白A（ConA）或佛波醇酯（PDB）加离子霉素（Ion）刺激下评价淋巴细胞增殖的模型, 通过流式细胞术分析Jagged 1/Fc对淋巴细胞增殖的作用; 采用碘化丙锭染色检测Jagged 1/Fc对PDB加Ion刺激的淋巴细胞周期变化的影响; 利用荧光标记的mAb双染技术观察Jagged 1/Fc对T细胞早期和中期活化标志CD69和CD25表达的影响。结果: Jagged 1/Fc对ConA刺激或非刺激的CD3+细胞CD69和CD25的表达无明显影响, 对ConA或PDB加Ion刺激或非刺激的淋巴细胞的增殖指数也无明显影响, 导致非刺激的淋巴细胞subG0期细胞百分比增高, S期细胞百分比降低, 但并不影响PDB加Ion诱导的淋巴细胞各期的变化。结论: 这些结果提示500 μg/L Jagged 1/Fc对小鼠淋巴细胞活化和增殖无明显作用, 对PDB加Ion刺激的淋巴细胞周期也无明显影响, 但能促进未受刺激的淋巴细胞凋亡, 使细胞停滞于G0/G1期, 阻止其进入S期。 【关键词】 Jagged 1 淋巴细胞 活化 增殖 细胞周期 　　[Abstract] AIM: To investigate effect of a soluble Jagged 1/Fc chimera protein (Jagged 1/Fc) on activation, proliferation and cell cycles of lymphocytes in BALB/c mice. METHODS: A model to evaluate the lymphocyte proliferation stimulated idyl ester (CFDASE)labeling technique. Under an effective dose of 500 μg/L of Jagged 1/Fc, the effect of it on the lymphocyte proliferation etry. A propidium iodidelabeling technique ate the influence of Jagged 1/Fc on the lymphocyte cellcycle stimulated by phorbol 12,13dibutyrate (PDB) plus Ionomycin (Ion). Expression levels of CD69 and CD25 molecules on surface of CD3+ lymphocytes activated onoclonal antibodylabeling technique. RESULTS: Jagged 1/Fc had no effect on the expression levels of CD69 and CD25 of the CD3+ lymphocytes stimulated phocytes stimulated by ConA or PDB plus Ion. It led to the increase of subG0 phase cell proportion and the decrease of S phase cell proportion of the lymphocytes, but did not influence the changes of the lymphocyte cellcycle induced by PDB plus Ion. CONCLUSION: The results suggest that Jagged 1/Fc has no obvious effect on the activation and proliferation of lymphocytes in mice, but may promote the apoptosis of them, cause the G0/G1 phase arrest and block the S phase entry. 　　[Keyphocyte; activation; proliferation; cell cycle