分子生物学讲稿分析.pptx

Title: Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein–protein interaction module Published: 2 Jul 2016 IF10;Part 1:Key words Part 2:Abstract Part 3:Results Part 4:Discussion;Part 1:Key words ;Here we describe a new tool for intracellular delivery of target proteins, named ‘exosomes for protein loading via optically reversible protein–protein interactions’ (EXPLORs). By integrating a reversible protein–protein interaction module controlled by blue light with the endogenous process of exosome biogenesis, we are able to successfully load cargo proteins into newly generated exosomes. Treatment with protein-loaded EXPLORs is shown to significantly increase intracellular levels of cargo proteins and their function in recipient cells in vitro and in vivo. These results clearly indicate the potential of EXPLORs as a mechanism for the efficient intracellular transfer of protein-based therapeutics into recipient cells and tissues. DOI: 10.1038/1;Part 2:Abstract ;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;1 May 2017;5、Schematic diagram of EXPLOR technology.;5、Schematic diagram of EXPLOR technology.