利用大孔树脂从葛根中分离纯化总黄酮_英文_刘火安分析.docVIP

Journa l o f Chinese Pha mr aceutica l Science s 2006, 15(2):121-126 121 Optmi ization of Preparative Separation and Purification of Total Flavonoids from Radix Puerariae by M acroporous Resin M ethod LIU H uo-an1, 2 , WANG Bo-chu1 , DA I Chuan-yun1* , SHAO Zhi-yong3 , HE Cong-lin1 , and JIA Yun2 (1. College of B ioeng ineering, Chongqing Un iversity, Chongqing 400030, China; 2. College of Chem istry and B iology, Chongqing Poly techn ic College, Chongqing 400050, Ch ina; 3. Interdepartm ent of Genetics, Iowa S ta te Un iversity, Iowa 50011 , USA) Abstract:A im To screen the optim um m acroporous resin and conditions fo r the isola tion and purification of flavonoids from Radix Puerariae. M ethods The static and dynam ic adso rption /desorp- tion m ethods w ere used, and the separa tion and purification process was evalua ted by m easuring the concentration of to tal flavonoid in the frac tions w ith UV spectropho tom e ter. R esults The SP70 m acro- porous re sin w as the m ost effective com pared w ith othe r m acroporous resins. The optim um cond itions w ere sc reened, which we re 0.5 g m L - 1 corresponding to crude d rug for concentra tion o f extrac t, pH 5 - 6, and appended 60 tim es the vo lum e of the resin bed (BV) w ith the adso rp tion speed 2 BV h- 1 , and the vo lum e o f aq. 70% (V /V) ethano l as e luan tw as 5 BV w ith desorption speed 2 BV h- 1. By th ism ethod, the final contents of tota l flavonoids exceeded 80%. Conclusion The SP70 m acropo rous resin is the m ost e ffective one fo r large-sca le iso lation and purification o f flavono ids from Rad ix Puerar- ia, w hich m ee ts industrial needs. K ey w ords:Radix Puerariae;to tal flavono ids;m acropo rous resin;separa tion;purification CLC number:R284. 2 D ocum ent code:A Article ID:1003-1057(2006)2-121-06  Introduction Pueraria lobata (W illd. ) Ohw i is a perennial le-gum inous plan t na tive to eastern A sia. The ex tract of Rad ix Puerariae is ri