不同年龄人骨髓间充质干细胞体外培养增殖的研究.pdfVIP

心脏杂志 (Chin Heart 1) 2∞8 ， 20 (1 ) • l3 • ·基础研究· 不同年龄人骨髓间充质干细胞体外培养增殖的研究 玉宇欢1 ，张浩2 ，李君2 ，朱沈军2 ，夏云峰1 ，张津津l (1.解放军总医院第一附属医院干部病房，北京 lC削37;2. 北京市阜外心血管病医院再生医学研究室) 摘要:目的 本研究分析不同年龄段人骨髓间充质干细胞的生长和增生情况，探讨年龄对于骨髓间充质干细胞的 影响。方法 采集61 例不同年龄人的骨髓，分为少年组、中青年组和老年组，分离间充质干细胞，进行体外培养， 观察其形态变化，对比分析其首次传代和每次传代时间、细胞克隆形成数量、细胞增长曲线的变化情况。结果 体 外培养的干细胞在形态上无年龄差异，均成梭形纤维状，早期呈克隆状生长。细胞的首次传代时间从少年到老年 呈逐渐增加的趋势，老年组明显长于另外两组;3 组的体外培养倍增时间无明显差异，但老年组的干细胞克隆数量 减少(差异尚未达到统计学意义) ，细胞的增殖显著低于另外两组(P 0. 05) 。结论 人骨髓间充质干细胞的生长 增殖能力随着增龄而逐渐减退，尤其在老年组呈现出更明显的变化。 关键词:骨髓间充质千细胞3 年龄;体外培养z 增殖性 中图分类号:R733. 7 ;R542. 2 文献标识码:A 文章编号:1侃9-7236(2∞8)OI -Ol3-04 Aging effect on growth and proliferation of human bone mesenchymal stem cells WANG Yu-meil , ZHANG Hao 2 , LI Jun 2 , ZHU Shen-j肌2 , XIA Yun-feni , Zhαng Jing-jing l (1. 304 Clinical Division , PLA General Hospital , Beijing 100037 , China; 2. Research Center for Cardi- ovascular Regenerative Medicine , Ministry of Health , Fu-Wai Heart Hospìtal , CAMS and PUMC , Bei- jing 1 ∞037 ， China) Abstract: AIM To determine the effect of aging on the replicative capacity of bone mesenchymal stem cells (BMSC) in different age groups. METHODS BMSCs were isolated from 61 subjects of different ages and were divided in 3 groups: teenage , middle age and advanced age. Cells were cultured in medi- um and different measurements were made to observe their proliferation. RESULTS There were no vis- ual differences in the shape and size in the first isolation between the 3 groups , but the old people experi- enced a litÙe faster gradual loss of spindle-formed features over cultivation and needed more time to reach the first