不同蛋白源酶解产物对过氧化氢诱导损伤PC12细胞保护作用研究.pdfVIP

现代食品科技 Modern Food Science and Technology 2016, Vol.32, No.10 不同蛋白源酶解产物对过氧化氢诱导损伤PC12细胞 的保护作用研究 李文治，徐巨才，苏国万，赵谋明 （华南理工大学轻工与食品学院，广东广州 510640） 摘要：本文以 H O 诱导损伤的大鼠肾上腺嗜铬细胞瘤 PC12 细胞为细胞模型，分别研究了三种蛋白源酶解产物（脑活素、鳕鱼 2 2 蛋白肽和核桃蛋白肽）对 H O 诱导 PC12 细胞氧化损伤的保护作用。结果表明，H O 的加入会使得 PC12 细胞的细胞数目减少，部 2 2 2 2 分胞体皱缩成圆形或椭圆形，周围光晕消失，且随着 H O 浓度的增加，细胞存活率逐渐降低。脑活素可获得最好的 PC12 细胞损伤 2 2 保护作用，核桃蛋白肽次之，而鳕鱼蛋白肽作用不明显；此外，结果表明脑活素、鳕鱼蛋白肽和核桃蛋白肽对受损伤细胞的存活和增 殖也能起一定的改善作用。因此，核桃蛋白肽是一种改善脑部功能性障碍的潜在原料。 关键词：PC12 细胞；脑活素；鳕鱼蛋白肽；核桃蛋白肽；氧化损伤 文章篇号：1673-9078(2016)10-22-27 DOI: 10.13982/j.mfst.1673-9078.2016.10.004 Neuroprotective Effects of Different Protein Hydrolysates against Oxidative Stress-induced Cytotoxicity in PC12 Cells LI Wen-zhi, XU Ju-cai, SU Guo-wan, ZHAO Mou-ming (College of Light Industry and Food Science, South China University of Technology, Guangzhou 510640, China) Abstract: Here, the effects of three protein hydrolysates (cerebrolysin, cod peptide, and walnut peptide) on rat pheochromocytoma (PC12) injured by H O was evaluated. The results showed that addition of H O decreased the number of viable PC12 cells and altered cell morphology, 2 2 2 2 resulting in shrunken circular or oval shapes accompanied by disappearance of the peripheral halo. However, cerebrolysin exhibited the strongest protective effects against H O -induced oxidative injury in PC12 cells, followed by walnut peptide, whereas no significant effect was observed 2 2 in the presence of cod peptide. Additionally, all th