control of differentiation of human mesenchymal stem cells by altering the geometry of nanofibers人类间充质干细胞分化的控制通过改变纳米纤维的几何.pdfVIP

Hindawi Publishing Corporation Journal of Nanotechnology Volume 2012, Article ID 429890, 9 pages doi:10.1155/2012/429890 Research Article Control of Differentiation of Human Mesenchymal Stem Cells by Altering the Geometry of Nanofibers Satoshi Fujita,1 Harue Shimizu,1 and Shin-ichiro Suye2 1 Department of Fibre Amenity Engineering, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan 2 Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui 910-8507, Japan Correspondence should be addressed to Satoshi Fujita, fujitas@u-fukui.ac.jp Received 30 April 2012; Accepted 21 May 2012 Academic Editor: A. M. Rao Copyright © 2012 Satoshi Fujita et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Effective differentiation of mesenchymal stem cells (MSCs) is required for clinical applications. To control MSC differentiation, induction media containing different types of soluble factors have been used to date; however, it remains challenging to obtain a uniformly differentiated population of an appropriate quality for clinical application by this approach. We attempted to develop nanofiber scaffolds for effective MSC differentiation by mimicking anisotropy of the extracellular matrix structure, to assess whether differentiation of these cells can be controlled by using geometrically different scaffolds. We evaluated MSC differentiation on aligned and random nanofibers, fabricated by electrospinning. We found that induction of MSCs into adipocytes was markedly more inhibited on random nanofibers than on aligned nanofibers. In addition, adipoinduction on align