formation of nanoscale bioimprints of muscle cells using uv-cured spin-coated polymers形成的纳米级bioimprints肌肉细胞使用所用spin-coated聚合物.pdfVIP

Hindawi Publishing Corporation Journal of Nanotechnology Volume 2009, Article ID 593410, 6 pages doi:10.1155/2009/593410 Research Article Formation of Nanoscale Bioimprints of Muscle Cells Using UV-Cured Spin-Coated Polymers Fahmi Samsuri,1 John S. Mitchell,2 Maan M. Alkaisi,1 and John J. Evans3 1 Department of Electrical and Computer Engineering, University of Canterbury, Christchurch 8140, New Zealand 2 The New Zealand Institute for Plant Food Research Ltd, Hamilton 3214, New Zealand 3 Centre for Neuroendocrinology and Christchurch School of Medicine and Health Sciences, University of Otago, Christchurch 8140, New Zealand Correspondence should be addressed to Maan M. Alkaisi, maan.alkaisi@canterbury.ac.nz Received 8 July 2009; Accepted 29 November 2009 Recommended by S. N. Piramanayagam We report a nanoscale replication method suitable for biological specimens that has potential in single cell studies and in formation of 3D biocompatible scaffolds. Earlier studies using a heat-curable polydimethylsiloxane (PDMS) or a UV-curable elastomer introduced Bioimprint replication to facilitate cell imaging. However, the replicating conditions for thermal polymerization are known to cause cell dehydration during curing. In this study, a UV-cured methacrylate copolymer was developed for use in creating replicas of living cells and was tested on rat muscle cells. Bioimprints of muscle cells were formed by spin coating under UV irradiation. The polymer replicas were then separated from the muscle cells and were analyzed under an Atomic Force Microscope (AFM), in tapping mode, because it has low tip-sample forces and thus will not destroy the fine structures of the imprint. The new polymer is biocompatible with higher replication resolution and has