calcium-induced aggregation of archaeal bipolar tetraether liposomes derived from the thermoacidophilic archaeon sulfolobus acidocaldariuscalcium-induced聚集的热点双四醚脂质体来源于thermoacidophilic archaeon硫化叶菌acidocaldarius.pdfVIP

Archaea 1, 175–183 © 2003 Heron Publishing—Victoria, Canada Calcium-induced aggregation of archaeal bipolar tetraether liposomes derived from the thermoacidophilic archaeon Sulfolobus acidocaldarius 1 2 3 1 ROBY KANICHAY, LAWRENCE T. BONI, PETER H. COOKE, TAPAN K. KHAN and 1,4 PARKSON LEE-GAU CHONG 1 Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA 19140, USA 2 Transave Inc., 11 Deer Park Drive, Suite 117, Monmouth, NJ 08852, USA 3 USDA, Eastern Regional Research Center, 600 East Mermaid Lane, Wyndmoor, PA 19038, USA 4 Author to whom correspondence should be addressed (pchong02@) Received November 18, 2002; accepted June 22, 2003; published online July 18, 2003 Summary Previously, we showed that the proton permeabil- PLFE contains a mixture of bipolar tetraether lipids with either ity of small unilamellar vesicles (SUVs) composed of polar a glycerol dialkyl calditol tetraether (GDNT; also called caldi- lipid fraction E (PLFE) from the thermoacidophilic archaeon toglycerocaldarchaeol) or a glycerol dialkyl glycerol tetra- Sulfolobus acidocaldarius was remarkably low and insensitive ether (GDGT; also called caldarchaeol) skeleton (Figure 1) to temperature (Komatsu and Chong 1998). In this study, we (Lo and Chang 1990, Sugai et al. 1995, Gliozzi et al. 2002). used photon correlation spectroscopy to investigate the time Both GDGT and GDNT have bisubstituted polar head groups dependence of PLFE SUV size as a function of Ca2+ concentra- and are thus designated bipolar tetraether lipids. Glycerol tion. In the absence