effects of splenectomy on spontaneously chronic pancreatitis in alyaly mice脾切除术的影响在alyaly小鼠自发慢性胰腺炎.pdfVIP

Hindawi Publishing Corporation Clinical and Developmental Immunology Volume 2010, Article ID 614890, 8 pages doi:10.1155/2010/614890 Research Article Effects of Splenectomy on Spontaneously Chronic Pancreatitis in aly/aly Mice Heng-Xiao Wang,1 Shuang-Qin Yi,2 Jun Li,1 Hayato Terayama,1 Munekazu Naito,1 Shuichi Hirai,1 Ning Qu,1 Nozomi Yi,1 and Masahiro Itoh1 1 Department of Anatomy, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan 2 Department of Anatomy and Neuroembryology, University of Kanazawa, Takara-machi 13-1, Kanazawa 920-8640, Japan Correspondence should be addressed to Shuang-Qin Yi, yixim@med.kanazawa-u.ac.jp Received 13 October 2009; Revised 4 January 2010; Accepted 16 January 2010 Academic Editor: Clelia M. Riera Copyright © 2010 Heng-Xiao Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Background and Aim. Mice with alymphoplasia (aly/aly) mutation characterized by a lack of lymph nodes, Peyer’s patches, and well-defined lymphoid follicles in the spleen were found. In this study, we used splenectomized aly/aly mice to elucidate the effects of secondary lymphoid organs in the development of aly/aly autoimmune pancreatitis. Methods. Forty-eight 10-week-old aly/aly mice were divided into two groups for splenectomy and sham operation. Histological and immunohistochemical analyses of the pancreas were performed at the ages of 20, 30, and 40 weeks old after operation, respectively. Results. Our results showed that mononuclear cell infiltration was restricted to the interlobular connective tissues at the age of 20 weeks, and not increase obviously