assessing genetic structure and relatedness of jerusalem artichoke (helianthus tuberosus l.) germplasm with rapd, issr and srap markers评估基因结构和亲缘洋姜(向日葵helianthus tuberosus l .).pdfVIP

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assessing genetic structure and relatedness of jerusalem artichoke (helianthus tuberosus l.) germplasm with rapd, issr and srap markers评估基因结构和亲缘洋姜(向日葵helianthus tuberosus l .).pdf

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assessing genetic structure and relatedness of jerusalem artichoke (helianthus tuberosus l.) germplasm with rapd, issr and srap markers评估基因结构和亲缘洋姜(向日葵helianthus tuberosus l .)

American Journal of Plant Sciences , 2011, 2, 753-764 753 doi:10.4236/ajps.2011.26090 Published Online December 2011 (http://www.SciRP.org/journal/ajps) Assessing Genetic Structure and Relatedness of Jerusalem Artichoke (Helianthus tuberosus L.) Germplasm with RAPD, ISSR and SRAP Markers 1* 1 2 1 Preeya Puangsomlee Wangsomnuk , Sudarat Khampa , Sanun Jogloy , Trin Srivong , Aran Patanothai2, Yong-Bi Fu3* 1Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, Thailand; 2Department of Plant Science and Agri- cultural Resources, Faculty of Agriculture, Khon Kaen University, Khon Kaen, Thailand; 3Plant Gene Resources of Canada, Saska- toon Research Centre, Agriculture and Agri-Food Canada, Saskatoon, Canada. * * Email: prepua@kku.ac.th, yong-bi.fu@agr.gc.ca th th st Received September 8 , 2011; revised October 7 , 2011; accepted October 21 , 2011. ABSTRACT Jerusalem artichoke (Helianthus tuberosus L.) is an old tuber crop with a recently renewed interest in multipurpose improvement, but little effort has been made to characterize its genetic resources. A study was conducted to assess ge- netic structure and genetic relatedness of 47 diverse Jerusalem artichoke accessions using RAPD, ISSR and SRAP markers. A total of 296 (87.1%) polymorphic bands were detected from 13 RAPD markers; 92 (80%) from six ISSR primers ; and 194 (88.6%) for nine combinations of SRAP primers. Five optimal clusters were inferred by the STRUC- TURE program from the RAPD or ISSR data, while six optimal clusters were found from the SRAP data or combined marker data. Significant linear relatio