assessment of transformed properties in vitro and of tumorigenicity in vivo in primary keratinocytes cultured for epidermal sheet transplantation评估改变属性的体外和体内致瘤性的主要角化细胞培养的表皮片移植.pdfVIP
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assessment of transformed properties in vitro and of tumorigenicity in vivo in primary keratinocytes cultured for epidermal sheet transplantation评估改变属性的体外和体内致瘤性的主要角化细胞培养的表皮片移植
Hindawi Publishing Corporation
Journal of Skin Cancer
Volume 2011, Article ID 936546, 8 pages
doi:10.1155/2011/936546
Research Article
Assessment of Transformed Properties In Vitro and of
Tumorigenicity In Vivo in Primary Keratinocytes Cultured for
Epidermal Sheet Transplantation
´ 1, 2 1 1 1 1 3 4
A. Thepot, A. Desanlis, E. Venet, L. Thivillier, V. Justin, A. P. Morel, F. DeFraipont, M.
Till,5 V. Krutovskikh,2 M. Tommasino,2 O. Damour,1 and P. Hainaut2
1 Banque de Tissus et Cellules, Hopital E. Herriot, 69437 Lyon cedex 03, France
ˆ
2 Centre International de Recherche sur le Cancer, 69372 Lyon cedex 08, France
3 Centre Leon Berard, 69373 Lyon cedex 03, France
´ ´
4 Centre Hospitalier Universitaire, 38043 Grenoble cedex 09, France
5 Laboratoire de Cytogenetique HFME, 69677 Bron, France
´ ´
´
Correspondence should be addressed to A. Thepot, thepot.amelie@
Received 30 June 2010; Accepted 28 August 2010
Academic Editor: Mark G. Lebwohl
´
Copyright © 2011 A. Thepot et al. This is an open access article distributed under the Creative Commons Attribution License,
which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Epidermal keratinocytes are used as a cell source for autologous and allogenic cell transplant therapy for skin burns. The question
addressed here is to determine whether the culture process may induce cellular, molecular
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