清热养阴活血法对糖尿病大鼠心肌细胞VEGF表达及超微结构影响.doc

清热养阴活血法对糖尿病大鼠心肌细胞VEGF表达及超微结构影响摘 要：目的：观察清热养阴活血方剂逐瘀化消饮对DM大鼠心脏VEGF表达和心肌细胞超微结构的影响。方法：采用β-cytotoxins大鼠模型，将Wistar大鼠随机分为正常组、模型组、胰岛素组、逐瘀化消饮低、中、高剂量+胰岛素组，观察糖尿病大鼠在胰岛素控制血糖基本一致的情况下，逐瘀化消饮治疗8周，用免疫组化方法检测DM大鼠心肌组织VEGF的分布状况，用H-600透射电镜观察心肌细胞的超微结构。结果：与胰岛素组相比，逐瘀化消饮能抑制DM大鼠心肌VEGF的表达，有效地保护心肌细胞的超微结构。结论：逐瘀化消饮通过多种机制来有效地抑制DM大鼠心脏微血管病变的发生发展，这可能是其防治DM大鼠心脏微血管病变发生发展的机理之一。 关键词：糖尿病；血管病变；实验研究；血管内皮生长因子；超微结构；逐瘀化消饮 中图分类号：R587.1文献标识码：A文章编号：1673-7717(2011)12-2753-04 Effect of Therapy of Clearing Heat, Nourishing Yin and Activating Blood on the Expression of Vascular Endothelial Growth Factor in Heart and Its Ultrastructure of Diabetic Rats DAI Hong (Chinese Medicine Department of Diagnostics,Hubei College of Traditional Chinese Medicine,Wuhan 430065,Hubei,China) Abstract:Objective:To observe the effect on the expression of vascular endothelial growth factor in heart and its ultrastructure of diabetic rats by method of clearing heat, nourishing yin and activating blood. Methods:The model rats of diabetes mellitus(DM) was induced by β-cytotoxins. Apart from the control groups, all the model rats were randomly divided into five groups,the pathology group, the insulin group, the low dose ZYHXY and insulin group(ZYHXY-L), the middle dose ZYHXY and insulin group(ZYHXY-M), the high dose ZYHXY and insulin group(ZYHXY-H). On the condition all the model rats had the same blood glucose controlled by insulin, observing the effect on the expression of VEGF in heart and its ultrastructure with electron microscope after treated eight weeks by ZYHXY. Results: In comparing with the insulin groups, the low, middle, high dose groups of ZYHXY have the significant difference(P＜0.05) in inhibiting the experession of VEGF in heart, and effectively protecting myocardiac ultrastructure. Conclusion: ZYHXY can prevent the development of angiopathy of heart through many aspects, which is probably one of treatment and prevention mechanisms of the diabetic angiopathy. Key words:Diabetes Mellitus；diabetic angio