a novel msdna (multicopy single-stranded dna) strain present in yersinia frederiksenii atcc 33641 contig01029 enteropathogenic bacteria with the genomic analysis of its retron英文论文.pdfVIP

SAGE-Hindawi Access to Research Journal of Pathogens Volume 2011, Article ID 693769, 6 pages doi:10.4061/2011/693769 Research Article A Novel msDNA (Multicopy Single-Stranded DNA) Strain Present in Yersinia frederiksenii ATCC 33641 Contig01029 Enteropathogenic Bacteria with the Genomic Analysis of It’s Retron Rasel Das,1 Tadashi Shimamoto,2 and Md. Arifuzzaman1 1 Department of Biochemistry and Biotechnology, University of Science and Technology Chittagong (USTC), Foy’s Lake, Chittagong 4202, Bangladesh 2 Laboratory of Food Microbiology and Hygiene, Graduate School of Biosphere Science, Hiroshima University, Higashi-Hiroshima, Hiroshima 739-8528, Japan Correspondence should be addressed to Md. Arifuzzaman, larif67@ Received 10 March 2011; Accepted 25 July 2011 Academic Editor: Dike O. Ukuku Copyright © 2011 Rasel Das et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Retron is a retroelement that encodes msDNA (multicopy single-stranded DNA) which was significantly found mainly in Gram- negative pathogenic bacteria. We screened Yersinia frederiksenii ATCC 33641 contig01029 for the presence of retroelement by using bioinformatics tools and characterized a novel retron-Yf79 on the chromosome that encodes msDNA-Yf79. In this study, we perceived that, the codon usage of retron-Yf79 were noteworthy different from those of the Y. frederiksenii genome. It demonstrates that, the retron-Yf79 was a foreign DNA element and integrated into this organism genome during their evolution. In addition to this, we have observed a transposase gene which is located just downstream of retron-Yf79. So, the enzyme might be r