complexation of eugenol (eg), as main component of clove oil and as pure compound, with β- and hp-β-cds络合的丁香酚(如),丁香油的主要成分,纯化合物,β-和hp-β-cds.pdfVIP

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complexation of eugenol (eg), as main component of clove oil and as pure compound, with β- and hp-β-cds络合的丁香酚(如),丁香油的主要成分,纯化合物,β-和hp-β-cds.pdf

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complexation of eugenol (eg), as main component of clove oil and as pure compound, with β- and hp-β-cds络合的丁香酚(如),丁香油的主要成分,纯化合物,β-和hp-β-cds

Food and Nutrition Sciences, 2012, 3, 716-723 /10.4236/fns.2012.36097 Published Online June 2012 (http://www.SciRP.org/journal/fns) Complexation of Eugenol (EG), as Main Component of Clove Oil and as Pure Compound, with β- and HP-β-CDs Pilar Hernández-Sánchez, Santiago López-Miranda, Carmen Lucas-Abellán, * Estrella Núñez-Delicado Departament of Food Technology and Nutrition, Catholic University of San Antonio, Murcia, Spain. * Email: enunez@ Received April 11th, 2011; revised July 17th, 2011; accepted August 6th, 2011 ABSTRACT Eugenol, both in its pure form (EG) and included in essential clove oil (CO) was successfully solubilized in aqueous solution by forming inclusion complexes with β-cyclodextrins (β-CDs) and its modified hydroxypropyl-β-CDs (HP-β-CDs). To investigate the molecular association between β-CDs/HP-β-CDs with pure EG and essential CO, phase solubility studies were undertaken. Essential CO formed insoluble complexes with β-CDs, but not with HP-β-CDs. The work clearly demonstrates complexes formation follow an order higher than 1:1 when high essential CO and β-CDs concentrations were used, however it was 1:1 in the case of essential CO-HP-β-CDs complexes. When pure EG was studied the results indicated that EG could form 1:1 inclusion complexes with β-CDs and HP-β-CDs. Based on the studies, the Kc values for pure EG were 4555 ± 225 M−1 and 10,633 ± 614 M−1 for β-CDs and HP-β-CDs, respectively, and 2005 ± 199 M−1 for essential CO-HP-β-CDs. These finding indicate that CDs are suitable for encapsulating EG. Keywords: Eugenol; Essential Clove Oil; β-CDs; HP-β-CDs 1. Introduction Eugenol (EG) (4-allyl-2-methoxyphenol) is the prin- cipal constituent of the essential CO, being 90% - 9