coupled tlc and maldi-tofms analyses of the lipid extract of the hyperthermophilic archaeon pyrococcus furiosus耦合的tlc和maldi-tofms分析脂质提取的hyperthermophilic archaeon海床furiosus.pdfVIP

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coupled tlc and maldi-tofms analyses of the lipid extract of the hyperthermophilic archaeon pyrococcus furiosus耦合的tlc和maldi-tofms分析脂质提取的hyperthermophilic archaeon海床furiosus.pdf

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coupled tlc and maldi-tofms analyses of the lipid extract of the hyperthermophilic archaeon pyrococcus furiosus耦合的tlc和maldi-tofms分析脂质提取的hyperthermophilic archaeon海床furiosus

Hindawi Publishing Corporation Archaea Volume 2012, Article ID 957852, 10 pages doi:10.1155/2012/957852 Research Article Coupled TLC and MALDI-TOF/MS Analyses of the Lipid Extract of the Hyperthermophilic Archaeon Pyrococcus furiosus Simona Lobasso,1 Patrizia Lopalco,2 Roberto Angelini,1 Rita Vitale,2 Harald Huber,3 ¨ 4 1, 5 Volker Muller, and Angela Corcelli 1 Department of Basic Medical Sciences, University of Bari Aldo Moro, Piazza G. Cesare, 70124 Bari, Italy 2 Institute for Microelectronics and Microsystems, IMM-CNR, National Research Council, 73100 Lecce, Italy 3 Institute for Microbiology and Archaeal Centre, University of Regensburg, 93053 Regensburg, Germany 4 Molecular Microbiology and Bioenergetics, Institute of Molecular Biosciences, Goethe University of Frankfurt, 60438 Frankfurt/Main, Germany 5 Institute for Chemical-Physical Processes, IPCF-CNR, National Research Council, 70126 Bari, Italy Correspondence should be addressed to Angela Corcelli, angela.corcelli@uniba.it Received 16 July 2012; Accepted 27 September 2012 Academic Editor: Yosuke Koga Copyright © 2012 Simona Lobasso et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The lipidome of the marine hyperthermophilic archaeon Pyrococcus furiosus was studied by means of combined thin-layer chromatography and MALDI-TOF/MS analyses of the total lipid extract. 80–90% of the major polar lipids were represented by archaeol lipids (diethers) and the remaining part by caldarchaeol lipids (tetraethers). The direct analysis of lipids