cytotoxic effect of the genus sinularia extracts on human scc25 and hacat cells细胞毒性的影响属sinularia提取物对人类scc25和hacat细胞.pdfVIP

Hindawi Publishing Corporation Journal of Toxicology Volume 2009, Article ID 634868, 8 pages doi:10.1155/2009/634868 Research Article Cytotoxic Effect of the Genus Sinularia Extracts on Human SCC25 and HaCaT Cells Guey-Horng Wang,1 Tzung-Han Chou,1 Rong-Jyh Lin,2 Jyh-Horng Sheu,3 Shih-Hao Wang,4 and Chia-Hua Liang1 1 Department of Cosmetic Science, Chia Nan University of Pharmacy and Science, Tainan 717, Taiwan 2 Department of Parasitology, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung 807, Taiwan 3 Department of Marine Biotechnology and Resources, National Sun Yat-Sen University, Kaohsiung 804, Taiwan 4 Graduate Institute of Pharmaceutical Chemistry, China Medical University, Taichung 404, Taiwan Correspondence should be addressed to Chia-Hua Liang, tinna ling@.tw Received 14 February 2008; Revised 2 June 2008; Accepted 4 July 2008 Recommended by Peter O’Brien Soft corals of the genus Sinularia are being increasingly adopted to treat a wide variety of disease processes. However, the mechanism underlying its activity against human oral cancer cells is poorly understood. This study evaluates the cyototoxicity effects of the genus Sinularia extracts (S. grandilobata, S. parva, S. triangula, S. scabra, S. nanolobata and S. gibberosa) by SCC25 and HaCaT cells. The cell adhesion assay indicates that extracts reduce the cell attachment. Extracts exhibit a dose-dependent cytotoxic effect using MTS assay.Treatment of extracts to observe the morphological alterations in cells, membrane blebbing, nuclear condensation, and apoptotic bodies is demonstrated. Flow cytometry shows that extracts sensitized the cells in the G0/ G1 and G /M phases with a concomitant significantly increased sub-G fraction, suggesting cell death by