effects of physical parameters on bacterial cell adsorption onto pre-imprinted sol-gel films物理参数对细菌细胞吸附的影响到pre-imprinted溶胶-凝胶法的电影.pdfVIP

Journal of Biomaterials and Nanobiotechnology, 2012, 3, 499-507 499 /10.4236/jbnb.2012.324051 Published Online October 2012 (http://www.SciRP.org/journal/jbnb) Effects of Physical Parameters on Bacterial Cell Adsorption onto Pre-Imprinted Sol-Gel Films 1 1 1 2 1* Jeanna Starosvetsky , Tally Cohen , Uta Cheruti , Dragoljub Bilanović, Robert Armon 1Faculty of Civil and Environmental Engineering Division of Environmental, Water and Agricultural Engineering, Technion, Haifa, Israel; 2Center for Environmental, Earth, and Space Studies, Bemidji State University, Bemidji, USA. * Email: cvrrobi@tx.technion.ac.il Received April 30th, 2012; revised June 15th, 2012; accepted July 16th, 2012 ABSTRACT Organically modified silica (ORMOSILS) thin films produced by sol-gel method were imprinted with two bacterial strains as whole cells in order to develop an easy, fast and specific probe to detect and specifically identify these micro- organisms when present in water samples. An important feature of the imprinting process was the molecular finger- prints left by these microorganisms alongside morphology, into imprinted film cavities. The films also showed high selectivity toward the imprinted template and were able to discriminate between two very close bacterial species (E. coli and S. typhimurium). In addition, several central physical parameters of the experimental water solution were examined (i.e., pH, ionic strength and the organic load exemplified by NaCl and TOC concentration, respectively). The method sensitivity to different bacterial concentrations was studied by confocal microscopy (CLSM) and quartz crystal micro- balance (QCM) tools. Results showed that increased bacterial conce